PUBLICATION

Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway

Authors
Lee, Y.H., Kawakami, K., HuangFu, W.C., Liu, I.H.
ID
ZDB-PUB-200403-244
Date
2020
Source
PLoS One   15: e0230943 (Journal)
Registered Authors
Kawakami, Koichi, Lee, Yen-Hua, Liu, I-Hsuan
Keywords
none
MeSH Terms
  • Animals
  • Antigens/metabolism*
  • Cell Polarity/physiology*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/physiology
  • Proteoglycans/metabolism*
  • RNA, Messenger/metabolism
  • Wnt Signaling Pathway/physiology*
  • Zebrafish/metabolism*
PubMed
32240230 Full text @ PLoS One
Abstract
Pericellular and extracellular proteoglycans play an important role in modulating morphogen gradients and signal transductions. Chondroitin sulfate proteoglycan 4 (Cspg4) is a membrane spanning proteoglycan expressed in immature progenitor cells and cancer cells. Cspg4 participates in cellular events such as proliferation, migration and signal transduction, and these events are generally important for embryo development. In this study, we characterized Cspg4 for its roles in zebrafish embryonic development. Our results demonstrated that cspg4 was maternally expressed from 0 to 3 hours post fertilization (hpf) and expressed in the anterior and posterior embryo end after 9 hpf. Knocking-down cspg4 resulted in a shorter anterior-posterior axis than control embryo, which could be rescued by co-injecting wnt11 mRNA suggesting that Cspg4 regulates body axis organization through modulating the Wnt/planar cell polarity signaling pathway. In addition, overexpressing cspg4 caused cyclopia. The Cspg4 transmembrane domain mutant embryo phenocopied the global over-expression of cspg4 mRNA and led to cyclopia with a very low penetrance. Our results demonstrated that the quantitatively and spatially accurate distribution of Cspg4 is critical for body axis and midline development during gastrulation.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes
Correction: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270539