PUBLICATION
Environmental estrogen exposure converts lipid metabolism in male fish to a female pattern mediated by AMPK and mTOR signaling pathways
- Authors
- Sun, S.X., Wu, J.L., Lv, H.B., Zhang, H.Y., Zhang, J., Limbu, S.M., Qiao, F., Chen, L.Q., Yang, Y., Zhang, M.L., Du, Z.Y.
- ID
- ZDB-PUB-200403-162
- Date
- 2020
- Source
- Journal of hazardous materials 394: 122537 (Journal)
- Registered Authors
- Du, Zhen-Yu, Limbu, Samwel Michele, Qiao, Fang
- Keywords
- 17β-estradiol (E2), Bisphenol A (BPA), Environmental estrogens, Feminization, Lipid metabolism
- MeSH Terms
-
- AMP-Activated Protein Kinases/metabolism
- Animals
- Benzhydryl Compounds/toxicity*
- Estradiol/toxicity*
- Estrogens, Non-Steroidal/toxicity*
- Female
- Feminization/chemically induced*
- Fishes
- Gonads/drug effects
- Lipid Metabolism/drug effects*
- Male
- Phenols/toxicity*
- Signal Transduction/drug effects*
- TOR Serine-Threonine Kinases/metabolism
- Transcription, Genetic/drug effects
- Water Pollutants, Chemical/toxicity
- PubMed
- 32203715 Full text @ J. Hazard. Mater.
Citation
Sun, S.X., Wu, J.L., Lv, H.B., Zhang, H.Y., Zhang, J., Limbu, S.M., Qiao, F., Chen, L.Q., Yang, Y., Zhang, M.L., Du, Z.Y. (2020) Environmental estrogen exposure converts lipid metabolism in male fish to a female pattern mediated by AMPK and mTOR signaling pathways. Journal of hazardous materials. 394:122537.
Abstract
Environmental estrogens, including bisphenol A (BPA) and 17β-estradiol (E2), which are widely used in industries and medicine, pose a severe ecological threat to fish due to feminization induction. However, the related metabolic basis for reproductive feminization in male fish has not been well addressed. We first found that female zebrafish exhibited higher lipid accumulation and lipogenesis activity than males. Next, we exposed male and female zebrafish to E2 (200 ng/L) or BPA (100 μg/L) for six weeks, and observed an early-phase reproductive feminization in males, accompanied with reduced spermatids, significant fat deposition and lipogenic gene expressions that mimicked female patterns. Cellular signaling assays revealed that, E2 or BPA modulated lipid metabolism in males mainly through lowering 5' AMP-activated protein kinase (AMPK) and upregulating the lipogenic mechanistic target of rapamycin (mTOR) pathways. For the first time, we show that environmental estrogens could alter lipid metabolism in male fish to a female pattern (metabolic feminization) prior to gonad feminization in male fish, to allows males to accumulate efficiently lipids to harmonize with the feminized gonads. This study suggests that negative effects of environmental estrogens, as hazardous materials, on vertebrate health are more complicated than originally thought.
Errata / Notes
This article is corrected by ZDB-PUB-220906-229 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping