PUBLICATION

Plasmid delivery by electroporation into fish skeletal muscle for recombinant protein secretion and uptake by oocytes

Authors
Iwaizumi, M., Yokoi, H., Suzuki, T.
ID
ZDB-PUB-200225-35
Date
2020
Source
Fish physiology and biochemistry   46(3): 1121-1130 (Journal)
Registered Authors
Suzuki, Tohru, Yokoi, Hayato
Keywords
Flounder, GFP, Goldfish, Luciferase, Ovary, Zebrafish
MeSH Terms
  • Animals
  • Electroporation*
  • Female
  • Flounder
  • Goldfish
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Growth Hormone/genetics
  • Growth Hormone/metabolism
  • Luciferases/genetics
  • Luciferases/metabolism
  • Muscle, Skeletal/metabolism*
  • Oocytes/metabolism*
  • Plasmids/administration & dosage*
  • Recombinant Proteins/metabolism*
  • Zebrafish/metabolism*
PubMed
32090288 Full text @ Fish Physiol. Biochem.
Abstract
We examined the efficiency of electroporation for the delivery of plasmid into the skeletal muscle and also examined the subsequent secretion of recombinant protein into the circulation system, using zebrafish, Japanese flounder, and bubble-eye goldfish. The expression area of GFP fluorescence was markedly expanded by electroporation. Introduced plasmid was retained in the muscle cells and continued to express GFP for at least 180 days in zebrafish, indicating the long lifespan of plasmid DNA in the muscle cell. Luciferase and a fusion of growth hormone (GH) and luciferase were secreted into the blood from muscle electroporated with CMV:secNluc and CMV:GH-Luc plasmids, respectively, indicating that recombinant proteins such as peptide hormones can be supplied to the blood by plasmid electroporation into muscle. Interestingly, luciferase activity was detected from fertilized eggs laid by zebrafish females that had been electroporated with CMV:secNluc, indicating that maturing oocytes incorporated recombinant protein from the blood stream that had been secreted from the muscle. The plasmid electroporation system reported here also may work for the delivery of recombinant proteins, such as Cas9, into the oocytes. Since the GH-Luc fusion protein was detected in the lymph of the eye-sac of bubble-eye goldfish, this fish may be useful for the production of recombinant protein.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes