PUBLICATION
Aqueous Synthesis of Triphenylphosphine-Modified Gold Nanoparticles for Synergistic In Vitro and In Vivo Photo-thermal Chemotherapy
- Authors
- Benyettou, F., Ramdas Nair, A., Dho, Y., Prakasam, T., Pasricha, R., Whelan, J., Traboulsi, H., Mazher, J., Sadler, K., Trabolsi, A.
- ID
- ZDB-PUB-200222-8
- Date
- 2020
- Source
- Chemistry (Weinheim an der Bergstrasse, Germany) 26(23): 5270-5279 (Journal)
- Registered Authors
- Sadler Edepli, Kirsten C.
- Keywords
- Apoptosis * Chemotherapy * Drug Delivery System * Hyperthermia * Photo-thermal Therapy * Triphenylphosphine AuNP * Zebrafish *
- MeSH Terms
-
- Antineoplastic Agents/chemistry
- Antineoplastic Agents/pharmacology*
- Gold/chemistry*
- HeLa Cells
- Humans
- Metal Nanoparticles/chemistry*
- Organophosphorus Compounds/chemistry*
- Phototherapy
- Polymers/chemistry
- Temperature
- PubMed
- 32077541 Full text @ Chemistry
Citation
Benyettou, F., Ramdas Nair, A., Dho, Y., Prakasam, T., Pasricha, R., Whelan, J., Traboulsi, H., Mazher, J., Sadler, K., Trabolsi, A. (2020) Aqueous Synthesis of Triphenylphosphine-Modified Gold Nanoparticles for Synergistic In Vitro and In Vivo Photo-thermal Chemotherapy. Chemistry (Weinheim an der Bergstrasse, Germany). 26(23):5270-5279.
Abstract
Triphenylphosphine (TPP) surface-functionalized and F-108 Pluronic-stabilized gold nanoparticles (F-108@TPP-AuNPs) have been synthesized via a one-step approach leading to well-defined (9.6 ± 1.6 nm) and water-soluble nanoparticles by microwave heating an aqueous solution of TPP-Au(I)Cl in the presence of a Pluronic polymer under basic conditions. TPP release was negligible under physiological conditions, but enhanced significantly at an acidic pH (5.4) mimicking that of a cancer cell. Laser irradiation (532 nm) raised the temperature of an aqueous solution of F-108@TPP-AuNPs to 51.7 °C within 5 minutes, confirming efficient light-to-heat conversion capabilities without significant photo-degradation. TEM confirmed intracellular localization of F-108@TPP-AuNPs in the cytosol, endosomes and lysosomes of HeLa cells. F-108@TPP-AuNPs were well tolerated by HeLa cells and zebrafish embryos at ambient temperatures and became toxic upon heat activation, suggesting synergistic interactions between heat and cytotoxic action by TPP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping