PUBLICATION

High-contrast, synchronous volumetric imaging with selective volume illumination microscopy

Authors
Truong, T.V., Holland, D.B., Madaan, S., Andreev, A., Keomanee-Dizon, K., Troll, J.V., Koo, D.E.S., McFall-Ngai, M.J., Fraser, S.E.
ID
ZDB-PUB-200216-12
Date
2020
Source
Communications biology   3: 74 (Journal)
Registered Authors
Fraser, Scott E.
Keywords
none
MeSH Terms
  • Animals
  • Brain/anatomy & histology
  • Brain/diagnostic imaging
  • Brain/ultrastructure
  • Decapodiformes/microbiology
  • Decapodiformes/ultrastructure
  • Heart/anatomy & histology
  • Heart/diagnostic imaging
  • Heart/physiology
  • Host Microbial Interactions/physiology
  • Image Processing, Computer-Assisted/instrumentation
  • Image Processing, Computer-Assisted/methods*
  • Imaging, Three-Dimensional/instrumentation
  • Imaging, Three-Dimensional/methods*
  • Larva
  • Light
  • Microscopy, Fluorescence/instrumentation
  • Microscopy, Fluorescence/methods
  • Organ Size
  • Seawater/microbiology
  • Video Recording/instrumentation
  • Video Recording/methods
  • Zebrafish
PubMed
32060411 Full text @ Commun Biol
Abstract
Light-field fluorescence microscopy uniquely provides fast, synchronous volumetric imaging by capturing an extended volume in one snapshot, but often suffers from low contrast due to the background signal generated by its wide-field illumination strategy. We implemented light-field-based selective volume illumination microscopy (SVIM), where illumination is confined to only the volume of interest, removing the background generated from the extraneous sample volume, and dramatically enhancing the image contrast. We demonstrate the capabilities of SVIM by capturing cellular-resolution 3D movies of flowing bacteria in seawater as they colonize their squid symbiotic partner, as well as of the beating heart and brain-wide neural activity in larval zebrafish. These applications demonstrate the breadth of imaging applications that we envision SVIM will enable, in capturing tissue-scale 3D dynamic biological systems at single-cell resolution, fast volumetric rates, and high contrast to reveal the underlying biology.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes