ZFIN ID: ZDB-PUB-200213-2
Evaluation of Antioxidant Activity of Spice-Derived Phytochemicals Using Zebrafish
Endo, Y., Muraki, K., Fuse, Y., Kobayashi, M.
Date: 2020
Source: International Journal of Molecular Sciences   21(3): (Journal)
Registered Authors: Endo, Yuka, Fuse, Yuji, Kobayashi, Makoto
Keywords: 6-MSITC, H2O2, NaAsO2, Nrf2, antioxidant activity, dietary phytochemical, food ingredient, oxidative stress, spice, zebrafish
MeSH Terms:
  • Allyl Compounds/pharmacology*
  • Animals
  • Antioxidants/pharmacology*
  • Arsenites/toxicity
  • Curcumin/pharmacology*
  • Drug Evaluation, Preclinical/methods*
  • High-Throughput Screening Assays/methods*
  • Hydrogen Peroxide/toxicity
  • Larva/drug effects
  • NF-E2-Related Factor 2/metabolism
  • Quercetin/pharmacology*
  • Spices*
  • Sulfides/pharmacology*
  • Zebrafish
  • Zebrafish Proteins/metabolism
PubMed: 32046157 Full text @ Int. J. Mol. Sci.
Various dietary phytochemicals seem to display antioxidant activity through the NF-E2-related factor 2 (Nrf2) pathway. However, few studies have demonstrated its antioxidant effect and Nrf2 dependency at the animal level. We constructed a zebrafish-based assay system to analyze the in vivo antioxidant activity of phytochemicals and examined the activity of 10 phytochemicals derived from spices, using this system as a pilot study. Hydrogen peroxide and arsenite were used as oxidative stressors, and Nrf2 dependency was genetically analyzed using an Nrf2-mutant zebrafish line. The activities of curcumin, diallyl trisulfide and quercetin were involved in the reduction of hydrogen peroxide toxicity, while those of cinnamaldehyde, isoeugenol and 6-(methylsulfinyl)hexyl isothiocyanate were involved in the reduction of arsenite toxicity. The antioxidant activities of these phytochemicals were all Nrf2 dependent, with the exception of cinnamaldehyde, which showed strong antioxidant effects even in Nrf2-mutant zebrafish. In summary, we succeeded in constructing an assay system to evaluate the in vivo antioxidant activity of various phytochemicals using zebrafish larvae. Using this system, we found that each spice-derived phytochemical has its own specific property and mechanism of antioxidant action.