PUBLICATION
Temporal and spatial expression of zebrafish mctp genes and evaluation of frameshift alleles of mctp2b
- Authors
- Espino-Saldaña, A.E., Durán-Ríos, K., Olivares-Hernandez, E., Rodríguez-Ortiz, R., Arellano-Carbajal, F., Martínez-Torres, A.
- ID
- ZDB-PUB-200201-3
- Date
- 2020
- Source
- Gene 738: 144371 (Journal)
- Registered Authors
- Keywords
- C2 domain, CRISPR/Cas9, Calcium sensors, Development
- MeSH Terms
-
- Zebrafish Proteins/genetics
- Animals
- Receptors, Calcium-Sensing/genetics
- CRISPR-Cas Systems
- Gene Expression Regulation, Developmental/genetics
- PubMed
- 32001375 Full text @ Gene
Abstract
MCTPs (multiple C2 domain proteins with two transmembrane regions) have been proposed as novel endoplasmic reticulum calcium sensors; however, their function remains largely unknown. Here we report the structure of the four mctp genes from zebrafish (mctp1a, mctp1b, mctp2a and mctp2b), their diversity, expression pattern during embryonic development and in adult tissue and the effect of knocking down the expression of Mctp2b by CRISPR/Cas9. The four mctp genes are expressed from early development and exhibit differential expression patterns but are found mainly in the nervous and muscular systems. Mctp2b tagged with fluorescent proteins and expressed in HEK-293 cells and neurons of the fish spinal cord localized mostly in the endoplasmic reticulum but also in lysosomes and late and recycling endosomes. Knocking down mctp2b expression impaired embryonic development, suggesting that the functional participation of this gene is relevant, at least during the early stages of development.
Genes / Markers
Figure Gallery (2 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping