PUBLICATION

Loss of Crb2b-lf leads to anterior segment defects in old zebrafish

Authors
Kujawski, S., Crespo, C., Luz, M., Yuan, M., Winkler, S., Knust, E.
ID
ZDB-PUB-200129-5
Date
2020
Source
Biology Open   9(2): (Journal)
Registered Authors
Crespo, Cátia, Knust, Elisabeth, Kujawski, Satu, Luz, Marta, Winkler, Sylke
Keywords
Cornea, Development, Iris, Lens, Polarity
MeSH Terms
  • Animals
  • Zebrafish Proteins/genetics*
  • Immunohistochemistry
  • Organogenesis/genetics*
  • Mutation
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Embryonic Development/genetics*
  • Membrane Proteins/genetics*
  • Homozygote
  • Eye/embryology
(all 12)
PubMed
31988089 Full text @ Biol. Open
Abstract
Defects in the retina or the anterior segment of the eye lead to compromised vision and affect millions of people. Understanding how these ocular structures develop and are maintained is therefore of paramount importance. The maintenance of proper vision depends, among others, on the function of genes controlling apico-basal polarity. In fact, mutations in polarity genes are linked to retinal degeneration in several species, including human. Here we describe a novel zebrafish crb2b allele (crb2be40 ), which specifically affects the crb2b long isoform. crb2be40 mutants are viable and display normal ocular development. However, old crb2be40 mutant fish develop multiple defects in structures of the anterior segment, which includes the cornea, the iris and the lens. Phenotypes are characterised by smaller pupils due to expansion of the iris and tissues of the iridocorneal angle, an increased number of corneal stromal keratocytes, an abnormal corneal endothelium and an expanded lens capsule. These findings illustrate a novel role for crb2b in the maintenance of the anterior segment and hence add an important function to this polarity regulator, which may be conserved in other vertebrates, including humans.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cbg16
    Point Mutation
    vu119TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      1 - 1 of 1
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      Mapping
      No data available