PUBLICATION
march5 Governs the Convergence and Extension Movement for Organization of the Telencephalon and Diencephalon in Zebrafish Embryos
- Authors
- Jung, J., Choi, I., Ro, H., Huh, T.L., Choe, J., Rhee, M.
- ID
- ZDB-PUB-200108-24
- Date
- 2020
- Source
- Molecules and cells 43(1): 76-85 (Journal)
- Registered Authors
- Huh, Tae-Lin, Ro, Hyunju
- Keywords
- March5/MITOL, convergence and extension movement, diencephalon, telencephalon, ubiquitin proteasome system
- MeSH Terms
-
- Telencephalon/embryology*
- Embryonic Development
- Mitochondria/metabolism*
- Gene Knockdown Techniques
- Humans
- Zebrafish/embryology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- HEK293 Cells
- Animals
- Homeostasis
- Central Nervous System/physiology*
- Diencephalon/embryology*
- Ubiquitination
- PubMed
- 31910335 Full text @ Mol. Cells
Citation
Jung, J., Choi, I., Ro, H., Huh, T.L., Choe, J., Rhee, M. (2020) march5 Governs the Convergence and Extension Movement for Organization of the Telencephalon and Diencephalon in Zebrafish Embryos. Molecules and cells. 43(1):76-85.
Abstract
MARCH5 is a RING finger E3 ligase involved in mitochondrial integrity, cellular protein homeostasis, and the regulation of mitochondrial fusion and fission. To determine the function of MARCH5 during development, we assessed transcript expression in zebrafish embryos. We found that march5 transcripts were of maternal origin and evenly distributed at the 1-cell stage, except for the mid-blastula transition, with expression predominantly in the developing central nervous system at later stages of embryogenesis. Overexpression of march5 impaired convergent extension movement during gastrulation, resulting in reduced patterning along the dorsoventral axis and alterations in the ventral cell types. Overexpression and knockdown of march5 disrupted the organization of the developing telencephalon and diencephalon. Lastly, we found that the transcription of march5 was tightly regulated by the transcriptional regulators CHOP, C/EBPα, Staf, Znf143a, and Znf76. These results demonstrate the essential role of March5 in the development of zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping