|ZFIN ID: ZDB-PUB-191226-6|
Genetic and functional implications of an exonic TRIM55 variant in heart failure
Heliste, J., Chheda, H., Paatero, I., Salminen, T.A., Akimov, Y., Paavola, J., Elenius, K., Aittokallio, T.
|Source:||Journal of Molecular and Cellular Cardiology 138: 222-233 (Journal)|
|Registered Authors:||Paatero, Ilkka|
|Keywords:||CRISPR/Cas9, Genetic variation, Heart failure, TRIM55, rs138811034|
|PubMed:||31866377 Full text @ J. Mol. Cell. Cardiol.|
Heliste, J., Chheda, H., Paatero, I., Salminen, T.A., Akimov, Y., Paavola, J., Elenius, K., Aittokallio, T. (2019) Genetic and functional implications of an exonic TRIM55 variant in heart failure. Journal of Molecular and Cellular Cardiology. 138:222-233.
Background To tackle the missing heritability of sporadic heart failure, we screened for novel heart failure-associated genetic variants in the Finnish population and functionally characterized a novel variant in vitro and in vivo.
Methods and results Heart failure-associated variants were screened in genotyping array data of the FINRISK study, consisting of 994 cases and 20,118 controls. Based on logistic regression analysis, a potentially damaging variant in TRIM55 (rs138811034), encoding an E140K variant, was selected for validations. In HL-1 cardiomyocytes, we used CRISPR/Cas9 technology to introduce the variant in the endogenous locus, and additionally TRIM55 wildtype or E140K was overexpressed from plasmid. Functional responses were profiled using whole-genome RNA sequencing, RT-PCR and Western analyses, cell viability and cell cycle assays and cell surface area measurements. In zebrafish embryos, cardiac contractility was measured using videomicroscopy after CRISPR-mediated knockout of trim55a or plasmid overexpression of TRIM55 WT or E140K. Genes related to muscle contraction and cardiac stress were highly regulated in Trim55 E140K/- cardiomyocytes. When compared to the WT/WT cells, the variant cells demonstrated reduced viability, significant hypertrophic response to isoproterenol, p21 protein overexpression and impaired cell cycle progression. In zebrafish embryos, the deletion of trim55a or overexpression of TRIM55 E140K reduced cardiac contractility as compared to embryos with wildtype genotype or overexpression of WT TRIM55, respectively.
Conclusions A previously uncharacterized TRIM55 E140K variant demonstrated a number of functional implications for cardiomyocyte functions in vitro and in vivo. These findings suggest a novel role for TRIM55 polymorphism in predisposing to heart failure.