PUBLICATION
A new method for vibration-based neurophenotyping of zebrafish
- Authors
- Wang, J., Wang, D., Hu, G., Yang, L., Yan, D., Wang, M., Serikuly, N., Alpyshov, E., Amstislavskaya, T.G., Demin, K.A., de Abreu, M.S., Zabegalov, K.N., Kalueff, A.V.
- ID
- ZDB-PUB-191223-24
- Date
- 2019
- Source
- Journal of Neuroscience Methods 333: 108563 (Journal)
- Registered Authors
- Kalueff, Allan V.
- Keywords
- behavioral analyses, drug screening, neurophenotyping, vibration signals, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal
- Locomotion
- Neurosciences*
- Vibration
- Zebrafish*
- PubMed
- 31863804 Full text @ J. Neurosci. Methods
Citation
Wang, J., Wang, D., Hu, G., Yang, L., Yan, D., Wang, M., Serikuly, N., Alpyshov, E., Amstislavskaya, T.G., Demin, K.A., de Abreu, M.S., Zabegalov, K.N., Kalueff, A.V. (2019) A new method for vibration-based neurophenotyping of zebrafish. Journal of Neuroscience Methods. 333:108563.
Abstract
Background The zebrafish (Danio rerio) is rapidly emerging as an important model species in neuroscience research. Neurobehavioral studies in zebrafish are typically based on automated video-tracking of individual or group fish responses to various stressors, drug treatments and genetic manipulations. However, moving zebrafish also emit vibration signals.
New method Here, we present the first evidence that vibration-based analyses can be used to assess zebrafish behaviors. Utilizing a free accelerometer smartphone application, we developed a simple inexpensive setup to detect vibration signals in adult zebrafish.
Results We demonstrate that moving zebrafish generate detectable, reproducible vibration power frequency spectra sensitive to various experimental manipulations, including sedative and anxiolytic treatments.
Comparison with existing methods The present study is the first report describing vibration-based behavioral characterization in zebrafish.
Conclusions The present proof-of-concept study expands the toolkit of zebrafish neurophenotyping methods to include vibration data, which may not only reflect changes in zebrafish locomotion, but can also eventually help detect more nuanced, behavior-specific changes in zebrafish phenotypes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping