PUBLICATION
Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish
- Authors
- Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M., Roeder, I., Scherf, N., Huisken, J.
- ID
- ZDB-PUB-191219-3
- Date
- 2019
- Source
- Nature communications 10: 5753 (Journal)
- Registered Authors
- Huisken, Jan, Reade, Anna
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement/physiology*
- Embryo, Nonmammalian/diagnostic imaging
- Embryo, Nonmammalian/embryology*
- Gastrulation/physiology*
- Germ Layers/diagnostic imaging
- Germ Layers/embryology*
- Imaging, Three-Dimensional/methods
- Intravital Microscopy/methods
- Multimodal Imaging/methods*
- Single-Cell Analysis/methods
- Time-Lapse Imaging/methods
- Zebrafish/embryology*
- PubMed
- 31848345 Full text @ Nat. Commun.
Citation
Shah, G., Thierbach, K., Schmid, B., Waschke, J., Reade, A., Hlawitschka, M., Roeder, I., Scherf, N., Huisken, J. (2019) Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish. Nature communications. 10:5753.
Abstract
The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in initial germlayer distributions are subsequently amplified by a global movement, which organizes the organ precursors along the embryonic body axis, giving rise to the blueprint of organ formation. The tools and data are available as a resource for the community.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping