PUBLICATION
Rapid and Efficient Live Zebrafish Embryo Genotyping
- Authors
- Zhang, X., Zhang, Z., Zhao, Q., Lou, X.
- ID
- ZDB-PUB-191219-15
- Date
- 2019
- Source
- Zebrafish 17(1): 56-58 (Journal)
- Registered Authors
- Lou, Xin
- Keywords
- basic protocol, genotyping, mutation, transgenes, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/enzymology
- Genotype*
- Genotyping Techniques/methods
- Genotyping Techniques/veterinary*
- Polymerase Chain Reaction/methods
- Zebrafish/genetics*
- PubMed
- 31851585 Full text @ Zebrafish
Citation
Zhang, X., Zhang, Z., Zhao, Q., Lou, X. (2019) Rapid and Efficient Live Zebrafish Embryo Genotyping. Zebrafish. 17(1):56-58.
Abstract
Most current methods for genotyping zebrafish embryos require sacrifice or raising the animal to at least 1 month of age for fin amputation. These limitations restrict the use of zebrafish and increase time and costs for experiments. This article introduces an innovative method for genotyping live zebrafish embryos. The method utilizes enzyme to extract a small amount of genetic material from the skin tissue of the embryo. Then, using conventional polymerase chain reaction (PCR) strategy, the embryo is genotyped. This approach was successful >95% of the time while maintaining high viability (>90%) of the embryo. This effective method can facilitate high-throughput screening and other applications in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping