PUBLICATION
Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol
- Authors
- de Carvalho, T.S., Cardoso, P.B., Santos-Silva, M., Lima-Bastos, S., Luz, W.L., Assad, N., Kauffmann, N., Passos, A., Brasil, A., Bahia, C.P., Moraes, S., Gouveia, A., de Jesus Oliveira Batista, E., Oliveira, K.R.M.H., Herculano, A.M.
- ID
- ZDB-PUB-191128-5
- Date
- 2019
- Source
- Oxidative medicine and cellular longevity 2019: 8419810 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Antioxidants/pharmacology
- Antioxidants/therapeutic use*
- Anxiety/chemically induced*
- Caffeine/adverse effects*
- Disease Models, Animal
- Female
- Oxidative Stress/drug effects*
- Zebrafish
- alpha-Tocopherol/pharmacology
- alpha-Tocopherol/therapeutic use*
- PubMed
- 31772712 Full text @ Oxid Med Cell Longev
Citation
de Carvalho, T.S., Cardoso, P.B., Santos-Silva, M., Lima-Bastos, S., Luz, W.L., Assad, N., Kauffmann, N., Passos, A., Brasil, A., Bahia, C.P., Moraes, S., Gouveia, A., de Jesus Oliveira Batista, E., Oliveira, K.R.M.H., Herculano, A.M. (2019) Oxidative Stress Mediates Anxiety-Like Behavior Induced by High Caffeine Intake in Zebrafish: Protective Effect of Alpha-Tocopherol. Oxidative medicine and cellular longevity. 2019:8419810.
Abstract
Anxiety is a common symptom associated with high caffeine intake. Although the neurochemical mechanisms of caffeine-induced anxiety remain unclear, there are some evidences suggesting participation of oxidative stress. Based on these evidences, the current study is aimed at evaluating the possible protective effect of alpha-tocopherol (TPH) against anxiety-like behavior induced by caffeine (CAF) in zebrafish. Adult animals were treated with CAF (100 mg/kg) or TPH (1 mg/kg)+CAF before behavioral and biochemical evaluations. Oxidative stress in the zebrafish brain was evaluated by a lipid peroxidation assay, and anxiety-like behavior was monitored using light/dark preference and novel tank diving test. Caffeine treatment evoked significant elevation of brain MDA levels in the zebrafish brain, and TPH treatment prevented this increase. Caffeine treatment also induced anxiety-like behavior, while this effect was not observed in the TPH+CAF group. Taken together, the current study suggests that TPH treatment is able to inhibit oxidative stress and anxiety-like behavior evoked by caffeine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping