PUBLICATION

Acute microcystin-LR exposure interfere thyroid hormones homeostasis in adult zebrafish (Danio rerio)

Authors
Gao, Y., Liu, Z., Jia, D., Hu, Q., Li, L., Tang, R., Li, D.
ID
ZDB-PUB-191118-7
Date
2019
Source
Chemosphere   243: 125258 (Journal)
Registered Authors
Gao, Yu
Keywords
Complement component 9, Iodothyronine deiodinases activity, Microcystin-LR, Thyroid hormones
MeSH Terms
  • Animals
  • Corticotropin-Releasing Hormone/genetics
  • Female
  • Homeostasis
  • Iodide Peroxidase/genetics
  • Male
  • Microcystins/toxicity*
  • Symporters
  • Thyroid Gland/metabolism
  • Thyroid Hormones/metabolism*
  • Thyroxine/metabolism
  • Toxicity Tests, Acute
  • Transcription, Genetic
  • Triiodothyronine/metabolism
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/metabolism
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism*
PubMed
31734598 Full text @ Chemosphere
Abstract
Microcystin-LR (MC-LR) in the aquatic environment may disturb thyroid hormone (TH) homeostasis. It is not clear how MC-LR affects downstream biological processes after TH disturbance. After exposure to 50, 100, 200 and 400 μg/L MC-LR for 24, 48, 72, or 96 h, alterations of the TH metabolism of adult zebrafish at thyroxine (T4), triiodothyronine (T3) levels, and iodothyronine deiodinase (Dio) activity, were observed. After exposure to MC-LR at 400 μg/L, T3 and T4 levels decreased significantly in females (p < 0.05) and returned to normal levels at 96 h. In males, T4 levels were not significantly different between groups. The expression of corticotropin releasing hormone, thyroid-stimulating hormone beta subunit, transthyretin, sodium/iodide cotransporter, thrombopoietin, thyroid hormone receptor alpha and beta changed, but not in a dose-dependent manner. Acute MC-LR exposure induced a negative feedback regulation of the hypothalamic-pituitary-thyroid axis in adult zebrafish, and females were more sensitive than males. In conclusion, acute MC-LR exposure disrupted the TH metabolism by altering Dio activity and gene expression of the HPT axis; these changes may affect the complement system through regulation of c9 mRNA synthesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping