PUBLICATION
Mechanical instabilities of aorta drive blood stem cell production: a live study
- Authors
- Poullet, N., Golushko, I., Lorman, V., Travnickova, J., Bureau, C., Chalin, D., Rochal, S., Parmeggiani, A., Kissa, K.
- ID
- ZDB-PUB-191117-8
- Date
- 2019
- Source
- Cellular and molecular life sciences : CMLS 77(17): 3453-3464 (Journal)
- Registered Authors
- Kissa-Marin, Karima
- Keywords
- 4D microscopy, Endothelial-to-haematopoietic transition, Haematopoiesis, Modeling, Zebrafish
- MeSH Terms
-
- Amides/pharmacology
- Aminoquinolines/pharmacology
- Animals
- Animals, Genetically Modified/metabolism
- Aorta/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Endothelial Cells/cytology
- Hematopoiesis
- Hematopoietic Stem Cells/cytology
- Hematopoietic Stem Cells/metabolism*
- Microscopy, Fluorescence
- Pyridines/pharmacology
- Pyrimidines/pharmacology
- Time-Lapse Imaging
- Zebrafish/growth & development
- PubMed
- 31732791 Full text @ Cell. Mol. Life Sci.
Citation
Poullet, N., Golushko, I., Lorman, V., Travnickova, J., Bureau, C., Chalin, D., Rochal, S., Parmeggiani, A., Kissa, K. (2019) Mechanical instabilities of aorta drive blood stem cell production: a live study. Cellular and molecular life sciences : CMLS. 77(17):3453-3464.
Abstract
During embryogenesis of all vertebrates, haematopoietic stem/progenitor cells (HSPCs) extrude from the aorta by a complex process named endothelial-to-haematopoietic transition (EHT). HSPCs will then colonize haematopoietic organs allowing haematopoiesis throughout adult life. The mechanism underlying EHT including the role of each aortic endothelial cell (EC) within the global aorta dynamics remains unknown. In the present study, we show for the first time that EHT involves the remodelling of individual cells within a collective migration of ECs which is tightly orchestrated, resulting in HSPCs extrusion in the sub-aortic space without compromising aorta integrity. By performing a cross-disciplinary study which combines high-resolution 4D imaging and theoretical analysis based on the concepts of classical mechanics, we propose that this complex developmental process is dependent on mechanical instabilities of the aorta preparing and facilitating the extrusion of HSPCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping