PUBLICATION

Interkingdom Genetic Mix-and-Match To Produce Novel Sunscreens

Authors
Osborn, A.R., Mahmud, T.
ID
ZDB-PUB-191116-1
Date
2019
Source
ACS synthetic biology   8: 2464-2471 (Journal)
Registered Authors
Keywords
Rhodococcus, Streptomyces, gadusol, gadusporine, mycosporine-like amino acid, sunscreen, zebra fish
MeSH Terms
  • Metabolic Engineering/methods*
  • Rhodococcus/genetics*
  • Sunscreening Agents/chemical synthesis*
  • Amino Acids/chemistry
  • Cyclohexanols/chemistry
  • Ultraviolet Rays
  • Zebrafish/genetics*
  • Skin Cream/chemistry
  • Anthozoa/drug effects
  • Streptomyces coelicolor/metabolism*
  • Animals
  • Plasmids/genetics
  • Benzophenones/adverse effects
  • Benzophenones/pharmacology
  • Streptomyces/genetics*
  • Cinnamates/adverse effects
  • Cinnamates/pharmacology
PubMed
31689088 Full text @ ACS Synth Biol
Abstract
Sunscreen-containing skincare products protect the skin from damage caused by sun exposure. However, many of them contain oxybenzone and/or octinoxate, which have been reported to be toxic to juvenile coral and to cause coral bleaching. Thus, there is a growing need for new sunscreen compounds that are less harmful to the environment. Here, we report an engineered biosynthetic pathway employing genes from a vertebrate and two Gram-(+) bacteria that forms novel sunscreen compounds with hybrid structures of gadusol and mycosporine-like amino acids, both of which are found in marine environments. These compounds, named gadusporines, have unique UV absorbance at 340 nm, expanding the range of mycosporine- and gadusol-based sunscreen products. The synthesis of gadusporines in Streptomyces coelicolor establishes a platform for the design and production of novel sunscreens.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping