PUBLICATION
Dorsal convergence of gastrula cells requires a Vangl2 and adhesion protein-dependent change in protrusive activity
- Authors
- Prince, D.J., Jessen, J.R.
- ID
- ZDB-PUB-191114-20
- Date
- 2019
- Source
- Development (Cambridge, England) 146(22): (Journal)
- Registered Authors
- Jessen, Jason R.
- Keywords
- Ezrin b, Fibronectin, Migration, Polarity, Protrusion, Vangl2
- MeSH Terms
-
- Animals
- Cadherins/metabolism
- Cell Adhesion
- Cell Movement
- Cell Polarity
- Crosses, Genetic
- Cytoskeletal Proteins/metabolism*
- Fibronectins/metabolism
- Gastrula/metabolism*
- Gastrulation*
- Green Fluorescent Proteins/metabolism
- Membrane Proteins/metabolism*
- Microscopy, Confocal
- Phenotype
- Pseudopodia/metabolism
- RNA, Messenger/metabolism
- Zebrafish/embryology
- Zebrafish Proteins/metabolism*
- PubMed
- 31719041 Full text @ Development
Citation
Prince, D.J., Jessen, J.R. (2019) Dorsal convergence of gastrula cells requires a Vangl2 and adhesion protein-dependent change in protrusive activity. Development (Cambridge, England). 146(22):.
Abstract
Lateral zebrafish hypoblast cells initiate dorsal convergence near mid-gastrulation and exhibit non-polarized morphologies, limited cell-cell contact, and indirect migration trajectories. By late gastrulation mesodermal cells become packed as they engage in planar cell polarity (PCP)-dependent movement. Here, we aimed to understand this transition in cell behavior by examining the relationship between protrusion dynamics and establishment of PCP and directed migration. We found that wild-type cells undergo a reduction in bleb protrusions near late gastrulation accompanied by a VANGL planar cell polarity protein 2 (Vangl2) regulated increase in filopodia number and polarization. Manipulation of blebs is sufficient to interfere with PCP and directed migration. We show that Vangl2, fibronectin, and Cadherin 2 function to suppress blebbing. Vangl2 maintains Ezrin b (Ezrb) protein levels and higher Ezrb activation rescues defective mediolateral cell alignment and migration paths in vangl2 mutant embryos. Transplantation experiments show that loss of vangl2 disrupts protrusion formation cell-autonomously while fibronectin acts non-autonomously. We propose that dorsal convergence requires the coordinated action of Vangl2, Ezrb, and cell adhesion proteins to inhibit blebs and promote polarized actin-rich protrusive activity and PCP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping