ZFIN ID: ZDB-PUB-191114-20
Dorsal convergence of gastrula cells requires a Vangl2 and adhesion protein-dependent change in protrusive activity
Prince, D.J., Jessen, J.R.
Date: 2019
Source: Development (Cambridge, England)   146(22): (Journal)
Registered Authors: Jessen, Jason R.
Keywords: Ezrin b, Fibronectin, Migration, Polarity, Protrusion, Vangl2
MeSH Terms:
  • Animals
  • Cadherins/metabolism
  • Cell Adhesion
  • Cell Movement
  • Cell Polarity
  • Crosses, Genetic
  • Cytoskeletal Proteins/metabolism*
  • Fibronectins/metabolism
  • Gastrula/metabolism*
  • Gastrulation*
  • Green Fluorescent Proteins/metabolism
  • Membrane Proteins/metabolism*
  • Microscopy, Confocal
  • Phenotype
  • Pseudopodia/metabolism
  • RNA, Messenger/metabolism
  • Zebrafish/embryology
  • Zebrafish Proteins/metabolism*
PubMed: 31719041 Full text @ Development
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ABSTRACT
Lateral zebrafish hypoblast cells initiate dorsal convergence near mid-gastrulation and exhibit non-polarized morphologies, limited cell-cell contact, and indirect migration trajectories. By late gastrulation mesodermal cells become packed as they engage in planar cell polarity (PCP)-dependent movement. Here, we aimed to understand this transition in cell behavior by examining the relationship between protrusion dynamics and establishment of PCP and directed migration. We found that wild-type cells undergo a reduction in bleb protrusions near late gastrulation accompanied by a VANGL planar cell polarity protein 2 (Vangl2) regulated increase in filopodia number and polarization. Manipulation of blebs is sufficient to interfere with PCP and directed migration. We show that Vangl2, fibronectin, and Cadherin 2 function to suppress blebbing. Vangl2 maintains Ezrin b (Ezrb) protein levels and higher Ezrb activation rescues defective mediolateral cell alignment and migration paths in vangl2 mutant embryos. Transplantation experiments show that loss of vangl2 disrupts protrusion formation cell-autonomously while fibronectin acts non-autonomously. We propose that dorsal convergence requires the coordinated action of Vangl2, Ezrb, and cell adhesion proteins to inhibit blebs and promote polarized actin-rich protrusive activity and PCP.
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