PUBLICATION

AHR-mediated oxidative stress contributes to the cardiac developmental toxicity of trichloroethylene in zebrafish embryos

Authors
Jin, H., Ji, C., Ren, F., Aniagu, S., Tong, J., Jiang, Y., Chen, T.
ID
ZDB-PUB-191110-3
Date
2019
Source
Journal of hazardous materials   385: 121521 (Journal)
Registered Authors
Ji, Cheng
Keywords
Aryl hydrocarbon receptor, Heart development, Reactive oxygen species, Trichloroethylene, Zebrafish embryos
MeSH Terms
  • Acetylcysteine/pharmacology
  • Animals
  • Azo Compounds/pharmacology
  • Cardiotoxicity/embryology
  • DNA Damage/drug effects
  • Embryo, Nonmammalian/drug effects*
  • Embryonic Development/drug effects*
  • Heart/embryology
  • Heart Defects, Congenital/chemically induced
  • Heart Defects, Congenital/embryology*
  • Heart Defects, Congenital/prevention & control
  • Oxidative Stress/drug effects
  • Purines/pharmacology
  • Pyrazoles/pharmacology
  • Reactive Oxygen Species/metabolism
  • Receptors, Aryl Hydrocarbon/antagonists & inhibitors
  • Receptors, Aryl Hydrocarbon/metabolism*
  • Trichloroethylene/toxicity*
  • Zebrafish
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/metabolism*
PubMed
31699484 Full text @ J. Hazard. Mater.
Abstract
Trichloroethylene (TCE), a widely used chlorinated solvent, is a common environmental pollutant. Current evidence shows that TCE could induce heart defects during embryonic development, but the underlining mechanism(s) remain unclear. Since activation of the aryl hydrocarbon receptor (AHR) could induce oxidative stress, we hypothesized that AHR-mediated oxidative stress may play a role in the cardiac developmental toxicity of TCE. In this study, we found that the reactive oxygen species (ROS) scavenger, N-Acetyl-L-cysteine (NAC), and AHR inhibitors, CH223191 (CH) and StemRegenin 1, significantly counteracted the TCE-induced heart malformations in zebrafish embryos. Moreover, both CH and NAC suppressed TCE-induced ROS and 8-OHdG (8-hydroxy-2' -deoxyguanosine). TCE did not affect ahr2 and cyp1a expression, but increased cyp1b1 expression, which was restored by CH supplementation. CH also attenuated the TCE-induced mRNA expression changes of Nrf2 signalling genes (nrf2b, gstp2, sod2, ho1, nqo1) and cardiac differentiation genes (gata4, hand2, c-fos, sox9b). In addition, the TCE enhanced SOD activity was attenuated by CH. Morpholino knockdown confirmed that AHR mediated the TCE-induced ROS and 8-OHdG generation in the heart of zebrafish embryos. In conclusion, our results suggest that AHR mediates TCE-induced oxidative stress, leading to DNA damage and heart malformations in zebrafish embryos.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping