PUBLICATION
Huntingtin confers fitness but is not embryonically essential in zebrafish development
- Authors
- Sidik, H., Ang, C.J., Pouladi, M.A.
- ID
- ZDB-PUB-191106-20
- Date
- 2019
- Source
- Developmental Biology 458(1): 98-105 (Journal)
- Registered Authors
- Keywords
- Huntingtin, Loss-of-function, Zebrafish
- MeSH Terms
-
- Animals
- Sequence Homology, Amino Acid
- Neurulation/genetics
- Gene Editing
- Body Size
- Genetic Fitness
- Morpholinos/pharmacology
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- CRISPR-Cas Systems
- Genetic Association Studies
- Humans
- Huntingtin Protein/chemistry
- Zebrafish Proteins/deficiency
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Gene Knockout Techniques
- Amino Acid Sequence
- Sequence Alignment
- Models, Animal*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/embryology
- Nerve Tissue Proteins/deficiency
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/physiology*
- Conserved Sequence
- PubMed
- 31682806 Full text @ Dev. Biol.
Citation
Sidik, H., Ang, C.J., Pouladi, M.A. (2019) Huntingtin confers fitness but is not embryonically essential in zebrafish development. Developmental Biology. 458(1):98-105.
Abstract
Attempts to constitutively knockout HTT in rodents resulted in embryonic lethality, curtailing efforts to study HTT function later in development. Here we show that HTT is dispensable for early zebrafish development, contrasting published zebrafish morpholino experiment results. Homozygous HTT knockouts were embryonically viable and appeared developmentally normal through juvenile stages. Comparison of adult fish revealed significant reduction in body size and fitness in knockouts compared to hemizygotes and wildtype fish, indicating an important role for wildtype HTT in postnatal development. Our zebrafish model provides an opportunity to understand the function of wildtype HTT later in development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping