PUBLICATION

The food preservative ethoxyquin impairs zebrafish development, behavior and alters gene expression profile

Authors
Pradhan, A., Bereketoglu, C., Martin, L., Duhagon, J., Olsson, P.E.
ID
ZDB-PUB-191106-1
Date
2019
Source
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association   135: 110926 (Journal)
Registered Authors
Pradhan, Ajay
Keywords
Development defects, Edema, Gene expression, Mortality, Toxicity
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/drug effects*
  • Embryonic Development/drug effects*
  • Ethoxyquin/toxicity*
  • Food Preservatives/toxicity*
  • Locomotion/drug effects
  • Transcriptome/drug effects*
  • Zebrafish/embryology*
PubMed
31676350 Full text @ Food Chem. Toxicol.
CTD
31676350
Abstract
In the present study, we investigated the detrimental effects of ethoxyquin (EQ) on zebrafish embryonic development using different endpoints including lethality, malformations, locomotion and gene expression. EQ is primarily used as a preservative in animal feed and it has been shown to have negative impacts on different laboratory animals. However, studies on the adverse effects of EQ in aquatic animals are still limited. In this study, zebrafish eggs were exposed to different concentrations of EQ ranging from 1 to 100 μM for six days. In the 100 μM treated groups 95 and 100% mortality was observed at 24 and 48 h, respectively. Delayed development, decreased pigmentation and pericardial edema were observed in larvae. Behavioral analysis of larvae demonstrated a distinct locomotive pattern in response to EQ both in light and dark indicating a possible developmental neurotoxicity and deficits in locomotion. The expression levels of genes involved in several physiological pathways including stress response, cell cycle and DNA damage were altered by EQ. Our results demonstrate that EQ could cause developmental and physiological toxicity to aquatic organisms. Hence, its toxic effect should be further analyzed and its use and levels in the environment must be monitored carefully.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping