PUBLICATION
Zebrafish, a model to develop nanotherapeutics that control neutrophils response during inflammation
- Authors
- García-López, J.P., Vilos, C., Feijóo, C.G.
- ID
- ZDB-PUB-191022-9
- Date
- 2019
- Source
- Journal of controlled release : official journal of the Controlled Release Society 313: 14-23 (Journal)
- Registered Authors
- Feijoo, Carmen G.
- Keywords
- Acute & chronic inflammation, Neutrophil recruitment, Polymeric nanoparticles, Zebrafish, p38 MAP Kinase
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents, Non-Steroidal/chemistry*
- Anti-Inflammatory Agents, Non-Steroidal/pharmacology
- Biocompatible Materials/chemistry*
- Cell Line
- Drug Compounding
- Drug Liberation
- Fluorescent Dyes/chemistry
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Imidazoles/chemistry*
- Imidazoles/pharmacology
- Inflammation/drug therapy*
- Larva/drug effects
- Models, Animal
- Nanocapsules/chemistry*
- Neutrophils/cytology
- Neutrophils/drug effects*
- Optical Imaging
- Phosphatidylethanolamines/chemistry
- Polyesters/chemistry
- Polyethylene Glycols/chemistry
- Protein Kinase Inhibitors/chemistry*
- Protein Kinase Inhibitors/pharmacology
- Pyridines/chemistry*
- Pyridines/pharmacology
- Signal Transduction
- Theranostic Nanomedicine
- Zebrafish
- p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors*
- PubMed
- 31622693 Full text @ J. Control Release
Citation
García-López, J.P., Vilos, C., Feijóo, C.G. (2019) Zebrafish, a model to develop nanotherapeutics that control neutrophils response during inflammation. Journal of controlled release : official journal of the Controlled Release Society. 313:14-23.
Abstract
Neutrophils are crucial modulators of the inflammation process, and their uncontrolled response worsens several chronic pathologies. The p38 mitogen-activated protein kinases (MAPKs) activity is critical for normal immune and inflammatory response through the regulation of pro-inflammatory cytokines synthesis. In this work, we study the effect of hybrid lipid-polymer nanoparticles loaded with the p38 MAPK inhibitor SB203580 in an acute and chronic inflammatory model in zebrafish containing a transgenic neutrophil cell line that constitutively expresses a green fluorescent protein. We identify the existence of at least two neutrophils subpopulation involved in the response during the acute inflammation triggered; a first-responder p38〈-independent subset and a second-responder p38〈-dependent subset. In the case of chronic inflammation, neutrophils recruited in the intestine only during the inflammation process, migrate in a p38〈-dependent manner. Likewise, we establish that SB203580-loaded in NPs exerts their action during at least a double period than the inhibitor administers directly in both types of inflammation. Our results demonstrate the exceptional potential of the zebrafish as an inflammatory model for studying novel nanotherapeutics that selectively inhibit the neutrophils response, and to identify functional neutrophils subpopulations involved in the inflammation process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping