PUBLICATION
Cellular reprogramming for successful CNS axon regeneration is driven by a temporally changing cast of transcription factors
- Authors
- Dhara, S.P., Rau, A., Flister, M.J., Recka, N.M., Laiosa, M.D., Auer, P.L., Udvadia, A.J.
- ID
- ZDB-PUB-191004-1
- Date
- 2019
- Source
- Scientific Reports 9: 14198 (Journal)
- Registered Authors
- Udvadia, Ava J.
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/metabolism
- Cellular Reprogramming/genetics*
- Central Nervous System/growth & development
- Central Nervous System/metabolism
- Humans
- Nerve Regeneration/genetics*
- Optic Nerve/growth & development
- Optic Nerve/pathology
- Optic Nerve Injuries/genetics
- Optic Nerve Injuries/pathology
- Recovery of Function/genetics
- Retinal Ganglion Cells/metabolism*
- Transcription Factors/genetics*
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 31578350 Full text @ Sci. Rep.
Citation
Dhara, S.P., Rau, A., Flister, M.J., Recka, N.M., Laiosa, M.D., Auer, P.L., Udvadia, A.J. (2019) Cellular reprogramming for successful CNS axon regeneration is driven by a temporally changing cast of transcription factors. Scientific Reports. 9:14198.
Abstract
In contrast to mammals, adult fish display a remarkable ability to fully regenerate central nervous system (CNS) axons, enabling functional recovery from CNS injury. Both fish and mammals normally undergo a developmental downregulation of axon growth activity as neurons mature. Fish are able to undergo damage-induced "reprogramming" through re-expression of genes necessary for axon growth and guidance, however, the gene regulatory mechanisms remain unknown. Here we present the first comprehensive analysis of gene regulatory reprogramming in zebrafish retinal ganglion cells at specific time points along the axon regeneration continuum from early growth to target re-innervation. Our analyses reveal a regeneration program characterized by sequential activation of stage-specific pathways, regulated by a temporally changing cast of transcription factors that bind to stably accessible DNA regulatory regions. Strikingly, we also find a discrete set of regulatory regions that change in accessibility, consistent with higher-order changes in chromatin organization that mark (1) the beginning of regenerative axon growth in the optic nerve, and (2) the re-establishment of synaptic connections in the brain. Together, these data provide valuable insight into the regulatory logic driving successful vertebrate CNS axon regeneration, revealing key gene regulatory candidates for therapeutic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping