PUBLICATION

Valves Are a Conserved Feature of the Zebrafish Lymphatic System

Authors
Shin, M., Nozaki, T., Idrizi, F., Isogai, S., Ogasawara, K., Ishida, K., Yuge, S., Roscoe, B., Wolfe, S.A., Fukuhara, S., Mochizuki, N., Deguchi, T., Lawson, N.D.
ID
ZDB-PUB-191001-3
Date
2019
Source
Developmental Cell   51(3): 374-386.e5 (Journal)
Registered Authors
Fukuhara, Shigetomo, Isogai, Sumio, Lawson, Nathan, Mochizuki, Naoki, Shin, Masahiro, Wolfe, Scot A., Yuge, Shinya
Keywords
lymphatic, lymphatic development, lymphatic valve, zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • Embryo, Nonmammalian/metabolism
  • Endothelial Progenitor Cells/metabolism
  • Endothelial Progenitor Cells/ultrastructure
  • Face/anatomy & histology
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/metabolism
  • Imaging, Three-Dimensional
  • Larva/anatomy & histology
  • Larva/metabolism
  • Lymphatic Vessels/anatomy & histology
  • Lymphatic Vessels/embryology*
  • Lymphatic Vessels/ultrastructure
  • Mice
  • Morphogenesis
  • Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/metabolism
PubMed
31564611 Full text @ Dev. Cell
Abstract
The lymphatic system comprises blind-ended tubes that collect interstitial fluid and return it to the circulatory system. In mammals, unidirectional lymphatic flow is driven by muscle contraction working in conjunction with valves. Accordingly, defective lymphatic valve morphogenesis results in backflow leading to edema. In fish species, studies dating to the 18th century failed to identify lymphatic valves, a precedent that currently persists, raising the question of whether the zebrafish could be used to study the development of these structures. Here, we provide functional and morphological evidence of valves in the zebrafish lymphatic system. Electron microscopy revealed valve ultrastructure similar to mammals, while live imaging using transgenic lines identified the developmental origins of lymphatic valve progenitors. Zebrafish embryos bearing mutations in genes required for mammalian valve morphogenesis show defective lymphatic valve formation and edema. Together, our observations provide a foundation from which to further investigate lymphatic valve formation in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes