PUBLICATION
Analysis of vertebrate vision in a 384-well imaging system
- Authors
- Thorn, R.J., Dombroski, A., Eller, K., Dominguez-Gonzalez, T.M., Clift, D.E., Baek, P., Seto, R.J., Kahn, E.S., Tucker, S.K., Colwill, R.M., Sello, J.K., Creton, R.
- ID
- ZDB-PUB-190929-13
- Date
- 2019
- Source
- Scientific Reports 9: 13989 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- none
- MeSH Terms
-
- Aminobenzoates/pharmacology
- Anesthetics/pharmacology
- Animals
- Anti-Anxiety Agents/pharmacology
- Behavior, Animal/drug effects
- Behavior, Animal/physiology*
- Diazepam/pharmacology
- Larva/drug effects
- Larva/physiology
- Software
- Swimming/physiology
- Vision, Ocular/drug effects
- Vision, Ocular/physiology*
- Zebrafish/physiology*
- PubMed
- 31562366 Full text @ Sci. Rep.
Citation
Thorn, R.J., Dombroski, A., Eller, K., Dominguez-Gonzalez, T.M., Clift, D.E., Baek, P., Seto, R.J., Kahn, E.S., Tucker, S.K., Colwill, R.M., Sello, J.K., Creton, R. (2019) Analysis of vertebrate vision in a 384-well imaging system. Scientific Reports. 9:13989.
Abstract
Visual impairment affects 253 million people worldwide and new approaches for prevention and treatment are urgently needed. While small molecules with potential beneficial effects can be examined in various model systems, the in vivo evaluation of visual function remains a challenge. The current study introduces a novel imaging system for measuring visually-guided behaviors in larval zebrafish. The imaging system is the first to image four 96-well plates with a single camera for automated measurements of activity in a 384-well format. In addition, it is the first system to project moving visual stimuli and analyze the optomotor response in the wells of a 96-well plate. We found that activity is affected by tricaine, diazepam and flumazenil. Surprisingly, diazepam treatments induce a loss of visual responses, at concentrations that do not affect activity or induce hyperactivity. Overall, our studies show that the developed imaging system is suitable for automated measurements of vertebrate vision in a high-throughput format.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping