PUBLICATION

lncrps25 play an essential role in motor neuron development through controlling the expression of olig2 in zebrafish

Authors
Gao, T., Li, J., Li, N., Gao, Y., Yu, L., Zhuang, S., Zhao, Y., Dong, X.
ID
ZDB-PUB-190925-13
Date
2019
Source
Journal of Cellular Physiology   235(4): 3485-3496 (Journal)
Registered Authors
Keywords
development, lncrps25, motor neuron, olig2, zebrafish
MeSH Terms
  • Animals
  • Cell Differentiation/genetics
  • Embryonic Development/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Humans
  • Morpholinos/genetics
  • Motor Neurons/metabolism*
  • Neurogenesis/genetics*
  • Oligodendrocyte Transcription Factor 2/genetics*
  • RNA, Long Noncoding/genetics*
  • Ribosomal Proteins/genetics*
  • Spinal Cord/growth & development
  • Spinal Cord/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics*
PubMed
31549395 Full text @ J. Cell. Physiol.
Abstract
lncrps25 is an intergenic long noncoding RNA (lncRNA), which is location close to rps25 (ribosomal protein S25) gene, is reported share high conserved sequence with NREP (neuronal regeneration-related protein) 3'-untranslated region. The function and mechanism of most of the lncRNA in embryo development remain largely unknown. In zebrafish, lncrps25 is widely expressed in the early embryonic stage and spinal cord during development. Morpholino (MO) knockdown of zebrafish lncrps25 exhibit locomotor behavior defects, caused by abnormal development of motor neurons. In addition, the defect of swimming ability and motor neurons could be recovery by microinject with lncrps25 RNA in lncrps25 morphants. By performing RNA sequencing and quantitative real-time polymerase chain reaction, we found that olig2 (oligodendrocyte transcription factor 2) messenger RNA (mRNA) was downregulated in lncrps25 morphants. Moreover, overexpression of olig2 mRNA in lncrps25 morphants partially rescued motor neurons development. Taken together, these results indicate that lncrps25 plays an essential role in the development of motor neurons in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes