PUBLICATION
Revealing sex-specific molecular changes in hypoxia-ischemia induced neural damage and subsequent recovery using zebrafish model
- Authors
- Das, T., Soren, K., Yerasi, M., Kumar, A., Chakravarty, S.
- ID
- ZDB-PUB-190914-8
- Date
- 2019
- Source
- Neuroscience letters 712: 134492 (Journal)
- Registered Authors
- Keywords
- Acute hypoxia, HIFs, TTC, TUNEL assay, sex-specific difference, stroke therapy
- MeSH Terms
-
- Hypoxia-Ischemia, Brain/genetics
- Hypoxia-Ischemia, Brain/metabolism*
- Hypoxia-Ischemia, Brain/physiopathology
- Zebrafish
- Exploratory Behavior/physiology*
- Cell Death/physiology
- Animals
- Recovery of Function/physiology*
- Motor Activity/physiology*
- Disease Models, Animal
- Brain/metabolism*
- Brain/physiopathology
- DNA Damage/physiology
- Female
- Male
- Swimming/physiology
- Sex Factors
- PubMed
- 31518677 Full text @ Neurosci. Lett.
Citation
Das, T., Soren, K., Yerasi, M., Kumar, A., Chakravarty, S. (2019) Revealing sex-specific molecular changes in hypoxia-ischemia induced neural damage and subsequent recovery using zebrafish model. Neuroscience letters. 712:134492.
Abstract
Functional recovery from hypoxia-ischemia depends on an individual's response to the ischemic damage and recovery. Many of the neurological disorders, including cerebral stroke have sex-specific characteristics. Deciphering the differential molecular mechanisms of sex-specific recovery from hypoxic-ischemic insult can improve medical practice in the treatment of cerebral stroke. In the present study, we describe the establishment of a sex-specific global hypoxia-ischemia neural damage and repair model in zebrafish. During hypoxic exposure a delayed behavioural response was observed in female fish that resumed normal swimming pattern earlier than their male counterparts. Moreover, female appeared more affected as they showed restricted locomotor and exploratory behaviour in novel tank test, reduced mitochondrial enzyme activity, enhanced DNA damage, and cell death after hypoxia insult. However, they showed a faster recovery as compared to male. Analysis of mRNA and protein expression levels of some characteristic hypoxic-ischemic markers showed notable sex-specific differences. Using zebrafish model, we have uncovered cellular and molecular differences in sex-specific systemic responses during the post-hypoxia recovery. This insight might help in devising better therapeutic strategy for stroke in female patients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping