PUBLICATION

Notch signaling restricts FGF pathway activation in parapineal cells to promote their collective migration

Authors
Wei, L., Al Oustah, A., Blader, P., Roussigné, M.
ID
ZDB-PUB-190910-11
Date
2019
Source
eLIFE   8: (Journal)
Registered Authors
Al Oustah, Amir, Blader, Patrick, Roussigné, Myriam
Keywords
FGF pathway, cell biology, collective cell migration, developmental biology, left right asymmetry, notch signaling, zebrafish
MeSH Terms
  • Zebrafish/embryology*
  • Signal Transduction*
  • Cell Movement*
  • Fibroblast Growth Factors/metabolism*
  • Epithalamus/embryology*
  • Animals
  • Gene Regulatory Networks
  • Gene Expression Regulation, Developmental
  • Receptors, Notch/metabolism*
(all 9)
PubMed
31498774 Full text @ Elife
Abstract
Coordinated migration of cell collectives is important during embryonic development and relies on cells integrating multiple mechanical and chemical cues. Recently, we described that focal activation of the FGF pathway promotes the migration of the parapineal in the zebrafish epithalamus. How FGF activity is restricted to leading cells in this system is, however, unclear. Here, we address the role of Notch signaling in modulating FGF activity within the parapineal. While Notch loss-of-function results in an increased number of parapineal cells activating the FGF pathway, global activation of Notch signaling decreases it; both contexts result in defects in parapineal migration and specification. Decreasing or increasing FGF signaling in a Notch loss-of-function context respectively rescues or aggravates parapineal migration defects without affecting parapineal cells specification. We propose that Notch signaling controls the migration of the parapineal through its capacity to restrict FGF pathway activation to a few leading cells.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ia12TgTransgenic Insertion
    kca3TgTransgenic Insertion
      pd3TgTransgenic Insertion
        pt6TgTransgenic Insertion
          ta52b
            		Point Mutation
            ti282a
              		Point Mutation
              zf246TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                rbpjaMO3-rbpjaMRPHLNO
                rbpjbMO1-rbpjbMRPHLNO
                rbpjbMO2-rbpjbMRPHLNO
                1 - 3 of 3
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                Fish
                No data available
                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                d2EGFPEFGd2EGFP
                DsRedEFGDsRed
                EGFPEFGEGFP
                GAL4EFGGAL4
                1 - 4 of 4
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                Mapping
                No data available
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                Citation
                Wei, L., Al Oustah, A., Blader, P., Roussigné, M. (2019) Notch signaling restricts FGF pathway activation in parapineal cells to promote their collective migration. eLIFE. 8:.