PUBLICATION
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
- Authors
- Britto, D.D., Hall, C.J., Astin, J.W.
- ID
- ZDB-PUB-190903-7
- Date
- 2019
- Source
- Journal of visualized experiments : JoVE (150): (Journal)
- Registered Authors
- Astin, Jonathan
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Line, Tumor
- Melanoma, Experimental/diagnostic imaging*
- Melanoma, Experimental/pathology
- Neoplasms/diagnostic imaging
- Neoplasms/pathology
- Neovascularization, Pathologic/diagnostic imaging*
- Neovascularization, Pathologic/pathology
- Time-Lapse Imaging/methods*
- Transplantation, Heterologous
- Xenograft Model Antitumor Assays/methods*
- Zebrafish
- PubMed
- 31475975 Full text @ J. Vis. Exp.
Citation
Britto, D.D., Hall, C.J., Astin, J.W. (2019) In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts. Journal of visualized experiments : JoVE. (150):.
Abstract
Tumor angiogenesis is a key target of anti-cancer therapy and this method has been developed to provide a new model to study this process in vivo. A zebrafish xenograft is created by implanting mammalian tumor cells into the perivitelline space of two days-post-fertilization zebrafish embryos, followed by measuring the extent of the angiogenic response observed at an experimental endpoint up to two days post-implantation. The key advantage to this method is the ability to accurately quantitate the zebrafish host angiogenic response to the graft. This enables detailed examination of the molecular mechanisms as well as the host vs tumor contribution to the angiogenic response. The xenografted embryos can be subjected to a variety of treatments, such as incubation with potential anti-angiogenesis drugs, in order to investigate strategies to inhibit tumor angiogenesis. The angiogenic response can also be live-imaged in order to examine more dynamic cellular processes. The relatively undemanding experimental technique, cheap maintenance costs of zebrafish and short experimental timeline make this model especially useful for the development of strategies to manipulate tumor angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping