PUBLICATION

Overexpression of Spexin 1 in the Dorsal Habenula Reduces Anxiety in Zebrafish

Authors
Jeong, I., Kim, E., Seong, J.Y., Park, H.C.
ID
ZDB-PUB-190903-4
Date
2019
Source
Frontiers in neural circuits   13: 53 (Journal)
Registered Authors
Jeong, Inyoung, Kim, Eunmi, Park, Hae-Chul
Keywords
anxiety, dorsal habenula, serotonergic system, spexin 1, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Anxiety/genetics
  • Anxiety/metabolism*
  • Gene Expression
  • Habenula/metabolism*
  • Interpeduncular Nucleus/metabolism*
  • Male
  • Peptide Hormones/biosynthesis*
  • Peptide Hormones/genetics
  • Zebrafish
PubMed
31474838 Full text @ Front. Neural Circuits
Abstract
Spexin (SPX) is an evolutionarily conserved neuropeptide that is expressed in the mammalian brain and peripheral tissue. Two orthologs are present in the teleost, SPX1 and SPX2. SPX1 is involved in reproduction and food intake. Recently, SPX1 neurons have been found to be located in the specific nuclei of dorsal habenula (dHb) and to project into the interpeduncular nucleus (IPN), in which galanin receptor 2a/2b (GALR2a/2b) expression was also observed. This indicates that habenula SPX1 neurons may interact with GALR2a/2b in the IPN; however, the function of SPX1 in the dHb-IPN neuronal circuit remains unknown. To determine the role of SPX1 in the dHb-IPN neural circuit, we generated transgenic zebrafish overexpressing SPX1 specifically in the dHb. We found that transgenic zebrafish overexpressing SPX1 in the dHb had anxiolytic behaviors compared with their wildtype siblings. Furthermore, quantitative PCR revealed that mRNA expression of galr2a and galr2b in the IPN and serotonin-related genes in the raphe was upregulated in the brains of transgenic zebrafish. Taken together, our data suggest that SPX1 function in the dHb-IPN neural circuits is implicated in the regulation of anxiety behaviors via modulation of the serotoninergic system in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes