Comparative analysis of the developmental toxicity in Xenopus laevis and Danio rerio induced by Al2 O3 nanoparticle exposure

Ismail, T., Lee, H.K., Kim, C., Kim, Y., Lee, H., Kim, J.H., Kwon, S., Huh, T.L., Khang, D., Kim, S.H., Choi, S.C., Lee, H.S.
Environmental toxicology and chemistry   38(12): 2672-2681 (Journal)
Registered Authors
Huh, Tae-Lin
Al2O3 nanoparticles, Danio rerio, Embryonic development, Toxicity, Xenopus laevis
MeSH Terms
  • Aluminum Oxide/metabolism
  • Aluminum Oxide/toxicity
  • Animals
  • Embryonic Development/drug effects*
  • Environmental Exposure
  • Female
  • Male
  • Nanoparticles/metabolism
  • Nanoparticles/toxicity*
  • Teratogens/metabolism
  • Teratogens/toxicity
  • Water Pollutants, Chemical/metabolism
  • Water Pollutants, Chemical/toxicity
  • Xenopus laevis/embryology*
  • Xenopus laevis/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
31470468 Full text @ Environ. Toxicol. Chem.
Engineered aluminum oxide nanoparticles (Al2 O3 NPs) having high-grade thermal stability and water dispersion properties are extensively used in different industries and personal care products. Toxicological response evaluation of these NPs is indispensable in assessing the health risks and exposure limits because of their industrial disposal into the aquatic environment. We assessed and compared the developmental toxicity of Al2 O3 NPs in Xenopus laevis and Danio rerio over a period of 96 h using the frog embryo teratogenic assay Xenopus and a fish embryo toxicity assay. Al2 O3 NP exposure produced dose-dependent embryonic mortality and decreased the embryo length, indicating a negative effect on growth. Moreover, Al2 O3 NPs induced various malformations such as small head size, a bent/deformed axis, edema, and gut malformation dose-dependently and altered the expression of heart- and liver-specific genes in both X. laevis and D. rerio, as revealed by whole-mount in-situ hybridization and reverse transcriptase polymerase chain reaction. In conclusion, the toxicological data suggest that Al2 O3 NPs are developmentally toxic and teratogenic and negatively affect the embryonic development of X. laevis and D. rerio. Our study can serve as a model for the toxicological evaluation of nanomaterial exposure on vertebrate development that is critical to ensure human and environmental safety. This article is protected by copyright. All rights reserved.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes