PUBLICATION
Hybrid genome assembly and annotation of Danionella translucida
- Authors
- Kadobianskyi, M., Schulze, L., Schuelke, M., Judkewitz, B.
- ID
- ZDB-PUB-190828-12
- Date
- 2019
- Source
- Scientific data 6: 156 (Other)
- Registered Authors
- Judkewitz, Benjamin
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain
- Cyprinidae/genetics*
- Gene Library
- Genome*
- Molecular Sequence Annotation*
- Neurons
- RNA-Seq
- Transcriptome
- PubMed
- 31451709 Full text @ Sci Data
Citation
Kadobianskyi, M., Schulze, L., Schuelke, M., Judkewitz, B. (2019) Hybrid genome assembly and annotation of Danionella translucida. Scientific data. 6:156.
Abstract
Studying neuronal circuits at cellular resolution is very challenging in vertebrates due to the size and optical turbidity of their brains. Danionella translucida, a close relative of zebrafish, was recently introduced as a model organism for investigating neural network interactions in adult individuals. Danionella remains transparent throughout its life, has the smallest known vertebrate brain and possesses a rich repertoire of complex behaviours. Here we sequenced, assembled and annotated the Danionella translucida genome employing a hybrid Illumina/Nanopore read library as well as RNA-seq of embryonic, larval and adult mRNA. We achieved high assembly continuity using low-coverage long-read data and annotated a large fraction of the transcriptome. This dataset will pave the way for molecular research and targeted genetic manipulation of this novel model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping