PUBLICATION
25-hydroxycholesterol impairs neuronal and muscular development in zebrafish
- Authors
- Jamadagni, P., Patten, S.A.
- ID
- ZDB-PUB-190827-3
- Date
- 2019
- Source
- Neurotoxicology 75: 14-23 (Journal)
- Registered Authors
- Patten, Shumoogum
- Keywords
- 25-hydroxycholesterol, behavior, neurodegeneration, neurodevelopment, oxysterol, zebrafish
- MeSH Terms
-
- Animals
- Cell Survival/drug effects
- Dose-Response Relationship, Drug
- Hydroxycholesterols/toxicity*
- Motor Activity/drug effects
- Motor Neurons/drug effects
- Movement/drug effects
- Muscle Development/drug effects*
- Neurogenesis/drug effects*
- Zebrafish/growth & development
- PubMed
- 31449834 Full text @ Neurotoxicology
Citation
Jamadagni, P., Patten, S.A. (2019) 25-hydroxycholesterol impairs neuronal and muscular development in zebrafish. Neurotoxicology. 75:14-23.
Abstract
Oxysterols have essential effects on brain homeostasis and their levels are often altered in neurodegenerative and neuroinflammatory diseases. Several studies have demonstrated the cytotoxic effects of 25-HC on different cell lines, however, not much is known about its effects on neurons in vivo. In this study, we examined the effects of 25-HC exposure on the nervous system development in the zebrafish. We showed that survival rate of zebrafish embryos/larvae is significantly decreased at doses of 25-HC above 40 μM. 25-HC was found to affect the motility of zebrafish larvae, primary motor axon and muscle morphology. Furthermore, larvae treated with 25-HC showed a reduced neuronal network and number of HuC-positive cells in the brain. An increased cell death was also observed in both the brain and spinal cord of zebrafish treated with 25-HC. Interestingly, administration of 25-HC at later stages of development (24 and 48 hours post fertilization) had no detrimental effects on motor axons. Altogether, our findings show that elevated levels of 25-HC may have important consequences on neuronal development and cell survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping