PUBLICATION

Glia-neuron interactions underlie state transitions to generalized seizures

Authors
Diaz Verdugo, C., Myren-Svelstad, S., Aydin, E., Van Hoeymissen, E., Deneubourg, C., Vanderhaeghe, S., Vancraeynest, J., Pelgrims, R., Cosacak, M.I., Muto, A., Kizil, C., Kawakami, K., Jurisch-Yaksi, N., Yaksi, E.
ID
ZDB-PUB-190826-16
Date
2019
Source
Nature communications   10: 3830 (Journal)
Registered Authors
Cosacak, Mehmet Ilyas, Jurisch-Yaksi, Nathalie, Kawakami, Koichi, Kizil, Caghan, Muto, Akira, Pelgrims, Robbrecht, Yaksi, Emre
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Brain/cytology
  • Brain/diagnostic imaging
  • Brain/physiopathology*
  • Cell Communication*
  • Cortical Excitability/physiology*
  • Disease Models, Animal
  • Epilepsy/physiopathology*
  • Gap Junctions/physiology
  • Glutamic Acid/metabolism
  • Humans
  • Microscopy, Confocal
  • Nerve Net/cytology
  • Nerve Net/physiopathology
  • Neuroglia/physiology
  • Neurons/physiology
  • Optical Imaging
  • Optogenetics
  • Patch-Clamp Techniques
  • Seizures/physiopathology*
  • Zebrafish
PubMed
31444362 Full text @ Nat. Commun.
Abstract
Brain activity and connectivity alter drastically during epileptic seizures. The brain networks shift from a balanced resting state to a hyperactive and hypersynchronous state. It is, however, less clear which mechanisms underlie the state transitions. By studying neural and glial activity in zebrafish models of epileptic seizures, we observe striking differences between these networks. During the preictal period, neurons display a small increase in synchronous activity only locally, while the gap-junction-coupled glial network was highly active and strongly synchronized across large distances. The transition from a preictal state to a generalized seizure leads to an abrupt increase in neural activity and connectivity, which is accompanied by a strong alteration in glia-neuron interactions and a massive increase in extracellular glutamate. Optogenetic activation of glia excites nearby neurons through the action of glutamate and gap junctions, emphasizing a potential role for glia-glia and glia-neuron connections in the generation of epileptic seizures.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes