PUBLICATION
Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system
- Authors
- Urasaki, A., Morishita, S., Naka, K., Uozumi, M., Abe, K., Huang, L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, T., Bessho, Y., Inagaki, N.
- ID
- ZDB-PUB-190823-4
- Date
- 2019
- Source
- Scientific Reports 9: 12156 (Journal)
- Registered Authors
- Bessho, Yasumasa, Inagaki, Naoyuki, Kawakami, Koichi, Matsui, Takaaki
- Keywords
- none
- MeSH Terms
-
- Carrier Proteins/classification
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Microscopy, Fluorescence
- Protein Binding
- Cell Movement
- Embryonic Development
- Neurons/physiology
- Phylogeny
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Organogenesis
- Lateral Line System/metabolism*
- Gene Editing
- Animals
- Actins/metabolism
- PubMed
- 31434971 Full text @ Sci. Rep.
Citation
Urasaki, A., Morishita, S., Naka, K., Uozumi, M., Abe, K., Huang, L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, T., Bessho, Y., Inagaki, N. (2019) Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system. Scientific Reports. 9:12156.
Abstract
The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping