PUBLICATION

Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system

Authors
Urasaki, A., Morishita, S., Naka, K., Uozumi, M., Abe, K., Huang, L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, T., Bessho, Y., Inagaki, N.
ID
ZDB-PUB-190823-4
Date
2019
Source
Scientific Reports   9: 12156 (Journal)
Registered Authors
Bessho, Yasumasa, Inagaki, Naoyuki, Kawakami, Koichi, Matsui, Takaaki
Keywords
none
MeSH Terms
  • Microscopy, Fluorescence
  • Neurons/physiology
  • Protein Binding
  • Lateral Line System/metabolism*
  • Phylogeny
  • Organogenesis
  • Carrier Proteins/classification
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism*
  • Actins/metabolism
  • Cell Movement
  • Embryonic Development
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
  • Gene Editing
(all 19)
PubMed
31434971 Full text @ Sci. Rep.
Abstract
The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15 to Prim-25
shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsLong-pec
shtn3ni102/ni102; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
shtn3ni102/ni102; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
shtn3ni102/ni102; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15 to Prim-25
shtn3ni102/ni102; nksaigff213aGt; nkuasgfp1aTgstandard conditionsLong-pec
shtn3ni102/ni102; shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
shtn3ni102/ni102; shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTgstandard conditionsPrim-15
1 - 10 of 13
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ni101
    		Complex
    ni102
      		Small Deletion
      nksaigff213aGtTransgenic Insertion
        nkuasgfp1aTgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          shtn1CRISPR1-shtn1CRISPR
          shtn1CRISPR2-shtn1CRISPR
          shtn3CRISPR1-shtn3CRISPR
          1 - 3 of 3
          Show
          Fish
          Fish
          nksaigff213aGt; nkuasgfp1aTg
          shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTg
          shtn3ni102/ni102; nksaigff213aGt; nkuasgfp1aTg
          shtn3ni102/ni102; shtn1ni101/ni101; nksaigff213aGt; nkuasgfp1aTg
          WT
          1 - 5 of 5
          Show
          Antibodies
          No data available
          Orthology
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GAL4FFEFGGAL4FF
          1 - 2 of 2
          Show
          Mapping
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          Citation
          Urasaki, A., Morishita, S., Naka, K., Uozumi, M., Abe, K., Huang, L., Watase, E., Nakagawa, O., Kawakami, K., Matsui, T., Bessho, Y., Inagaki, N. (2019) Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system. Scientific Reports. 9:12156.