PUBLICATION
Chronic exposure to copper oxide nanoparticles causes muscle toxicity in adult zebrafish
- Authors
- Mani, R., Balasubramanian, S., Raghunath, A., Perumal, E.
- ID
- ZDB-PUB-190808-12
- Date
- 2019
- Source
- Environmental science and pollution research international 27(22): 27358-27369 (Journal)
- Registered Authors
- Azhwar, Raghunath
- Keywords
- Antioxidants, Chronic exposure, CuO-NPs, Muscle damage, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Copper
- Metal Nanoparticles*
- Nanoparticles*
- Oxidative Stress
- Oxides
- Zebrafish
- PubMed
- 31388954 Full text @ Environ. Sci. Pollut. Res. Int.
Citation
Mani, R., Balasubramanian, S., Raghunath, A., Perumal, E. (2019) Chronic exposure to copper oxide nanoparticles causes muscle toxicity in adult zebrafish. Environmental science and pollution research international. 27(22):27358-27369.
Abstract
Repeated deposition of copper oxide nanoparticles (CuO-NPs) into aquatic systems makes them a global threat since the NPs accumulate in various organs of the fish particularly skeletal muscle. In the present study, adult zebrafish were exposed to different concentrations of CuO-NPs (1 and 3 mg/L) for a period of 30 days. The status of functional markers (acetylcholinesterase, creatine kinase-MB, and lactate dehydrogenase) and oxidative stress markers (oxidants and antioxidants) were analyzed. The histological changes in muscle were studied followed by the immunohistochemistry expression for catalase. Further, the expression of myoD, myogenin, pax7, β-actin, and desmin was examined by semi-quantitative reverse transcriptase polymerase chain reaction. The results indicated that chronic exposure to CuO-NPs causes muscular damage as evidenced by elevated levels of functional markers. There was a significant increase in the oxidants with reduction in the antioxidant levels, implying that the antioxidant enzymes were unable to scavenge the free radicals induced by the CuO-NPs. The histopathological analysis showed degeneration and atrophy in the treated groups confirming muscle damage. The immunohistochemical catalase expression in the muscle was reduced in the treated groups further supporting the evidence that the antioxidant has suffered a decline. The altered gene expression indicates skeletal muscle damage due to the CuO-NPs exposure. Overall, the data suggest that chronic exposure to CuO-NPs caused muscular toxicity which may lead to muscle degeneration in adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping