PUBLICATION

Amphioxus functional genomics and the origins of vertebrate gene regulation

Authors
Marlétaz, F., Firbas, P.N., Maeso, I., Tena, J.J., Bogdanovic, O., Perry, M., Wyatt, C.D.R., de la Calle-Mustienes, E., Bertrand, S., Burguera, D., Acemel, R.D., van Heeringen, S.J., Naranjo, S., Herrera-Ubeda, C., Skvortsova, K., Jimenez-Gancedo, S., Aldea, D., Marquez, Y., Buono, L., Kozmikova, I., Permanyer, J., Louis, A., Albuixech-Crespo, B., Le Petillon, Y., Leon, A., Subirana, L., Balwierz, P.J., Duckett, P.E., Farahani, E., Aury, J.M., Mangenot, S., Wincker, P., Albalat, R., Benito-Gutiérrez, È., Cañestro, C., Castro, F., D'Aniello, S., Ferrier, D.E.K., Huang, S., Laudet, V., Marais, G.A.B., Pontarotti, P., Schubert, M., Seitz, H., Somorjai, I., Takahashi, T., Mirabeau, O., Xu, A., Yu, J.K., Carninci, P., Martinez-Morales, J.R., Crollius, H.R., Kozmik, Z., Weirauch, M.T., Garcia-Fernàndez, J., Lister, R., Lenhard, B., Holland, P.W.H., Escriva, H., Gómez-Skarmeta, J.L., Irimia, M.
ID
ZDB-PUB-190806-2
Date
2018
Source
Nature   564: 64-70 (Journal)
Registered Authors
Bertrand, Stéphanie, Bogdanovic, Ozren, Cañestro-García, Cristian, D'Aniello, Salvatore, de la Calle-Mustienes, Elisa, Duckett, Paul, Escriva, Hector, Gómez-Skarmeta, José Luis, Irimia, Manuel, Laudet, Vincent, Maeso, Ignacio, Martinez-Morales, Juan R., Mirabeau, Olivier, Naranjo, Silvia, Permanyer, Jon, Schubert, Michael, Takahashi, Tokiharu, Tena, Juan
Keywords
none
Datasets
GEO:GSE106428, GEO:GSE106431, GEO:GSE115945, GEO:GSE106430
MeSH Terms
  • Animals
  • Body Patterning/genetics
  • DNA Methylation
  • Gene Expression Regulation*
  • Genomics*
  • Humans
  • Lancelets/embryology
  • Lancelets/genetics*
  • Molecular Sequence Annotation
  • Promoter Regions, Genetic
  • Transcriptome/genetics
  • Vertebrates/genetics*
PubMed
30464347 Full text @ Nature
Abstract
Vertebrates have greatly elaborated the basic chordate body plan and evolved highly distinctive genomes that have been sculpted by two whole-genome duplications. Here we sequence the genome of the Mediterranean amphioxus (Branchiostoma lanceolatum) and characterize DNA methylation, chromatin accessibility, histone modifications and transcriptomes across multiple developmental stages and adult tissues to investigate the evolution of the regulation of the chordate genome. Comparisons with vertebrates identify an intermediate stage in the evolution of differentially methylated enhancers, and a high conservation of gene expression and its cis-regulatory logic between amphioxus and vertebrates that occurs maximally at an earlier mid-embryonic phylotypic period. We analyse regulatory evolution after whole-genome duplications, and find that-in vertebrates-over 80% of broadly expressed gene families with multiple paralogues derived from whole-genome duplications have members that restricted their ancestral expression, and underwent specialization rather than subfunctionalization. Counter-intuitively, paralogues that restricted their expression increased the complexity of their regulatory landscapes. These data pave the way for a better understanding of the regulatory principles that underlie key vertebrate innovations.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes