PUBLICATION
Novel fluorescent probe with a bridged Si-O-Si bond for the reversible detection of hypochlorous acid and biothiol amino acids in live cells and zebrafish
- Authors
- Zhang, Y., Zuo, Y., Yang, T., Gou, Z., Wang, X., Lin, W.
- ID
- ZDB-PUB-190716-7
- Date
- 2019
- Source
- The Analyst 144(17): 5075-5080 (Journal)
- Registered Authors
- Zhang, Yu
- Keywords
- none
- MeSH Terms
-
- Animals
- Cysteine/analysis*
- Cysteine/chemistry
- Fluorescent Dyes/chemistry*
- Glutathione/analysis*
- Glutathione/chemistry
- HeLa Cells
- Homocysteine/analysis*
- Homocysteine/chemistry
- Humans
- Hypochlorous Acid/analysis*
- Limit of Detection
- Mice
- Microscopy, Confocal/methods
- Microscopy, Fluorescence/methods
- Molecular Structure
- Naphthalimides/chemistry*
- Oxidation-Reduction
- RAW 264.7 Cells
- Zebrafish
- PubMed
- 31305811 Full text @ Analyst
Citation
Zhang, Y., Zuo, Y., Yang, T., Gou, Z., Wang, X., Lin, W. (2019) Novel fluorescent probe with a bridged Si-O-Si bond for the reversible detection of hypochlorous acid and biothiol amino acids in live cells and zebrafish. The Analyst. 144(17):5075-5080.
Abstract
Herein, we report the design of a novel fluorescent probe consisting of a naphthalimide fluorophore and a silicone small molecule for the reversible detection of hypochlorous acid and biothiol amino acids. The response mechanism of BSi-1 is based on the concept of the S-based oxidation/reduction. The probe was found to be suitable for imaging HOCl in HeLa, RAW 264.7 cells and zebrafish, demonstrating its utility in biological applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping