PUBLICATION

Neural signatures of sleep in zebrafish

Authors
Leung, L.C., Wang, G.X., Madelaine, R., Skariah, G., Kawakami, K., Deisseroth, K., Urban, A.E., Mourrain, P.
ID
ZDB-PUB-190712-13
Date
2019
Source
Nature   571: 198-204 (Journal)
Registered Authors
Kawakami, Koichi, Mourrain, Philippe
Keywords
none
MeSH Terms
  • Animals
  • Biological Evolution
  • Brain/cytology
  • Brain/drug effects
  • Brain/physiology
  • Brain/physiopathology
  • Ependyma/cytology
  • Eye Movements
  • Fluorescence
  • Heart Rate
  • Hypnotics and Sedatives/pharmacology
  • Hypothalamic Hormones/metabolism
  • Melanins/metabolism
  • Neurons/drug effects
  • Neurons/physiology*
  • Pigmentation/physiology
  • Pituitary Hormones/metabolism
  • Polysomnography/methods
  • Sleep/drug effects
  • Sleep/physiology*
  • Sleep Deprivation/physiopathology
  • Sleep, REM/drug effects
  • Sleep, REM/physiology
  • Sleep, Slow-Wave/drug effects
  • Sleep, Slow-Wave/physiology
  • Zebrafish/physiology*
PubMed
31292557 Full text @ Nature
Abstract
Slow-wave sleep and rapid eye movement (or paradoxical) sleep have been found in mammals, birds and lizards, but it is unclear whether these neuronal signatures are found in non-amniotic vertebrates. Here we develop non-invasive fluorescence-based polysomnography for zebrafish, and show-using unbiased, brain-wide activity recording coupled with assessment of eye movement, muscle dynamics and heart rate-that there are at least two major sleep signatures in zebrafish. These signatures, which we term slow bursting sleep and propagating wave sleep, share commonalities with those of slow-wave sleep and paradoxical or rapid eye movement sleep, respectively. Further, we find that melanin-concentrating hormone signalling (which is involved in mammalian sleep) also regulates propagating wave sleep signatures and the overall amount of sleep in zebrafish, probably via activation of ependymal cells. These observations suggest that common neural signatures of sleep may have emerged in the vertebrate brain over 450 million years ago.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes