PUBLICATION

Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis

Authors
Sharma, P., Saraswathy, V.M., Xiang, L., Fürthauer, M.
ID
ZDB-PUB-190712-12
Date
2019
Source
Scientific Reports   9: 9958 (Journal)
Registered Authors
Fürthauer, Maximilian
Keywords
none
MeSH Terms
  • Neural Tube/cytology
  • Neural Tube/embryology*
  • Intracellular Signaling Peptides and Proteins/metabolism*
  • Spinal Cord/cytology
  • Spinal Cord/embryology*
  • Membrane Proteins/metabolism*
  • Zebrafish Proteins/metabolism*
  • Neural Stem Cells/cytology
  • Neural Stem Cells/metabolism
  • Zebrafish/embryology*
  • Cell Differentiation
  • Animals
  • Receptors, Notch/metabolism*
  • Neurogenesis/physiology*
(all 14)
PubMed
31292468 Full text @ Sci. Rep.
Abstract
The morphogenesis of the nervous system requires coordinating the specification and differentiation of neural precursor cells, the establishment of neuroepithelial tissue architecture and the execution of specific cellular movements. How these aspects of neural development are linked is incompletely understood. Here we inactivate a major regulator of embryonic neurogenesis - the Delta/Notch pathway - and analyze the effect on zebrafish central nervous system morphogenesis. While some parts of the nervous system can establish neuroepithelial tissue architecture independently of Notch, Notch signaling is essential for spinal cord morphogenesis. In this tissue, Notch signaling is required to repress neuronal differentiation and allow thereby the emergence of neuroepithelial apico-basal polarity. Notch-mediated suppression of neurogenesis is also essential for the execution of specific morphogenetic movements of zebrafish spinal cord precursor cells. In the wild-type neural tube, cells divide at the organ midline to contribute one daughter cell to each organ half. Notch signaling deficient animals fail to display this behavior and therefore form a misproportioned spinal cord. Taken together, our findings show that Notch-mediated suppression of neurogenesis is required to allow the execution of morphogenetic programs that shape the zebrafish spinal cord.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
chi3
    		Small Deletion
    ta52b
      		Point Mutation
      tfi91
        		Point Mutation
        tr233
          		Point Mutation
          um14TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            dlaMO1-dlaMRPHLNO
            mib1MO1-mib1MRPHLNO
            rbpjaMO6-rbpjaMRPHLNO
            rbpjbMO6-rbpjbMRPHLNO
            tp53MO4-tp53MRPHLNO
            1 - 5 of 5
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            Fish
            No data available
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            1 - 1 of 1
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            Mapping
            No data available
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            Citation
            Sharma, P., Saraswathy, V.M., Xiang, L., Fürthauer, M. (2019) Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis. Scientific Reports. 9:9958.