PUBLICATION
Cloning, molecular characterization, and tissue differential expression of connective tissue growth factor (ctgf) of grass carp
- Authors
- Pan, W.Q., Wang, J.P., Tu, Z.H., Gan, T., Hu, J., Wei, J., Leng, X.J., Li, X.Q.
- ID
- ZDB-PUB-190704-5
- Date
- 2019
- Source
- Fish physiology and biochemistry 45(4): 1431-1443 (Journal)
- Registered Authors
- Hu, Jing
- Keywords
- Gene clone, Grass carp, RACE, Tissue differential expression, ctgf
- MeSH Terms
-
- Animals
- Carps/genetics*
- Cloning, Molecular
- Connective Tissue Growth Factor/blood
- Connective Tissue Growth Factor/genetics*
- DNA, Complementary/genetics
- Fish Proteins/blood
- Fish Proteins/genetics*
- Gene Expression
- Spleen/metabolism
- PubMed
- 31267430 Full text @ Fish Physiol. Biochem.
Citation
Pan, W.Q., Wang, J.P., Tu, Z.H., Gan, T., Hu, J., Wei, J., Leng, X.J., Li, X.Q. (2019) Cloning, molecular characterization, and tissue differential expression of connective tissue growth factor (ctgf) of grass carp. Fish physiology and biochemistry. 45(4):1431-1443.
Abstract
Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by rapid amplification of cDNA ends (RACE) method; then, the characteristics of this gene and the predicted protein sequence were analyzed by bioinformatics methods, and the tissue differential expression pattern was detected by the quantitative real-time PCR. The results showed that the grass carp ctgf gene has a full-length of 2223 bp, encoding 343 amino acids. The deduced CTGF protein is a hydrophilic and secretary protein with a molecular mass of 37,978.2 Da and an isoelectric point of 8.22. The signal peptide locates between residue positions 1 and 22 of the polypeptide chain. The protein contains α-helix, β-strand, and loops. The CTGF protein of grass carp shows a homology of 98%, 96%, 91%, and 91% with Wuchang bream (Megalobrama amblycephala), zebrafish (Danio rerio), common carp (Cyprinus carpio), and Mexican tetra (Astyanax mexicanus). The grass carp ctgf gene expressed significantly higher in blood and spleen than that in other tissues (P < 0.05). The low expression tissues included the heart, gill, skin, muscle, kidney, brain, and intestinal, and the lowest expression tissue was the liver. The results are consistent with the function of this gene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping