PUBLICATION

Dual roles of hydrogen peroxide in promoting zebrafish renal repair and regeneration

Authors
Chen, J., Yu, T., He, X., Fu, Y., Dai, L., Wang, B., Wu, Y., He, J., Li, Y., Zhang, F., Zhao, J., Liu, C.
ID
ZDB-PUB-190701-3
Date
2019
Source
Biochemical and Biophysical Research Communications   516(3): 680-685 (Journal)
Registered Authors
He, Jianbo, Li, Yang
Keywords
Acute kidney injury, Hydrogen peroxide, Kidney regeneration, Renal injury repair
MeSH Terms
  • Acute Kidney Injury/genetics
  • Acute Kidney Injury/metabolism*
  • Animals
  • Apoptosis/physiology
  • Cell Proliferation/physiology
  • Disease Models, Animal*
  • Humans
  • Hydrogen Peroxide/antagonists & inhibitors
  • Hydrogen Peroxide/metabolism*
  • Kidney/metabolism
  • Kidney/pathology
  • Kidney/physiopathology
  • Kidney Tubules, Proximal/metabolism*
  • Kidney Tubules, Proximal/pathology
  • Morpholinos
  • NADPH Oxidases/antagonists & inhibitors
  • NADPH Oxidases/genetics
  • NADPH Oxidases/metabolism
  • Regeneration/physiology
  • Signal Transduction/physiology*
  • Zebrafish
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
31248596 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Acute renal injury (AKI) is a serious disorder of renal failure or renal damage that occurs within hours or days. At present, there is no approved pharmaceutical treatment for AKI. Zebrafish is an excellent model for studying the repair of AKI because of its remarkable ability to repair kidney injury. Using zebrafish AKI model inducing by gentamicin, we found that hydrogen peroxide (H2O2) plays dual roles during the period of AKI recovery including renal repair and kidney regeneration. In the repair stage of AKI, H2O2 was produced in proximal and distal segments of renal tubules. By inhibiting H2O2 generation using Duox Vivo-Morpholino or chemical inhibitor, it was observed of severe damage of renal tubules, and extensive cell apoptosis. In the stage of regeneration, we found that H2O2 was highly generated in renal interstitium. Inhibiting production of H2O2 could significantly down-regulate the ability of kidney regeneration, which was associated with the failure of proliferation of renal progenitor cells. Therefore, H2O2 acts as a protective factor in renal repair and an initial signal of kidney regeneration, indicating the key roles of H2O2 in promoting recovery of AKI in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes