PUBLICATION
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity
- Authors
- Tan, X., Xu, P., Zhang, Y., Zhang, P.J.
- ID
- ZDB-PUB-190701-28
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 236: 110310 (Journal)
- Registered Authors
- Tan, Xungang, Zhang, Yuqing
- Keywords
- Myogenesis, Olive flounder, Somites, Zebrafish, mrf4 promoter
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Embryonic Development
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Flounder/embryology
- Flounder/genetics*
- Gene Expression Regulation, Developmental
- Humans
- Muscles/metabolism*
- Myogenic Regulatory Factors/chemistry
- Myogenic Regulatory Factors/genetics*
- Organ Specificity
- Promoter Regions, Genetic/genetics*
- PubMed
- 31255700 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Tan, X., Xu, P., Zhang, Y., Zhang, P.J. (2019) Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 236:110310.
Abstract
Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping