PUBLICATION

Fibroblast growth factor signaling mediates progenitor cell aggregation and nephron regeneration in the adult zebrafish kidney

Authors
Gallegos, T.F., Kamei, C.N., Rohly, M., Drummond, I.A.
ID
ZDB-PUB-190621-11
Date
2019
Source
Developmental Biology   454(1): 44-51 (Journal)
Registered Authors
Drummond, Iain
Keywords
Adult kidney stem cell, Fibroblast growth factor, Kidney, Nephron, Regeneration
MeSH Terms
  • Kidney/cytology
  • Kidney/metabolism
  • Zebrafish Proteins/metabolism
  • Receptor, Fibroblast Growth Factor, Type 1/metabolism
  • Animals
  • Organogenesis
  • Adult Stem Cells/metabolism
  • Nephrons/metabolism*
  • Cell Aggregation/physiology
  • Signal Transduction/physiology
  • Fibroblast Growth Factors/metabolism*
  • Regeneration/physiology
  • Zebrafish/metabolism
  • Stem Cells/cytology*
  • Stem Cells/metabolism
(all 15)
PubMed
31220433 Full text @ Dev. Biol.
Abstract
The zebrafish kidney regenerates after injury by development of new nephrons from resident adult kidney stem cells. Although adult kidney progenitor cells have been characterized by transplantation and single cell RNA seq, signals that stimulate new nephron formation are not known. Here we demonstrate that fibroblast growth factors and FGF signaling is rapidly induced after kidney injury and that FGF signaling is required for recruitment of progenitor cells to sites of new nephron formation. Chemical or dominant negative blockade of Fgfr1 prevented formation of nephron progenitor cell aggregates after injury and during kidney development. Implantation of FGF soaked beads induced local aggregation of lhx1a:EGFP  + kidney progenitor cells. Our results reveal a previously unexplored role for FGF signaling in recruitment of renal progenitors to sites of new nephron formation and suggest a role for FGF signaling in maintaining cell adhesion and cell polarity in newly forming kidney epithelia.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidney, chemical treatment by environment: tert-butyl groupAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidney, chemical treatment by environment: tert-butyl groupAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
AB/TUchemical treatment by injection: gentamicin sulfate, chemical ablation: kidneyAdult
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pd1TgTransgenic Insertion
    pt303TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      AB/TU
      pd1Tg
      pt303Tg
      WT
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      Antibodies
      No data available
      Orthology
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      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
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      Mapping
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      Citation
      Gallegos, T.F., Kamei, C.N., Rohly, M., Drummond, I.A. (2019) Fibroblast growth factor signaling mediates progenitor cell aggregation and nephron regeneration in the adult zebrafish kidney. Developmental Biology. 454(1):44-51.