PUBLICATION

Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae

Authors
Jelcic, M., Enyedi, B., Niethammer, P.
ID
ZDB-PUB-190608-9
Date
2019
Source
Methods in molecular biology (Clifton, N.J.)   1982: 283-299 (Chapter)
Registered Authors
Enyedi, Balázs, Niethammer, Philipp
Keywords
Biosensor, Diffusion, Gradient, Hydrogen peroxide, Intravital, Quantitative, Zebrafish
MeSH Terms
  • Animals
  • Biosensing Techniques
  • Hydrogen Peroxide/metabolism*
  • Image Processing, Computer-Assisted
  • Larva
  • Molecular Imaging*/methods
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Signal Transduction
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
31172479 Full text @ Meth. Mol. Biol.
Citation
Jelcic, M., Enyedi, B., Niethammer, P. (2019) Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae. Methods in molecular biology (Clifton, N.J.). 1982:283-299.
Abstract
Quantitative aspects of extracellular H2O2 signaling in animals, such as its spatiotemporal dynamics within tissues, remain little understood. Here we detail an optimized, experimental setup for measuring the dynamics and physiological consequences of extracellular H2O2 application to live tissues by intravital biosensor imaging in zebrafish larvae.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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