PUBLICATION
Alleviation of Pb2+ pollution-induced oxidative stress and toxicity in microglial cells and zebrafish larvae by chicoric acid
- Authors
- Mu, Y., Yu, J., Ji, W., Chen, L., Wang, X., Yan, B.
- ID
- ZDB-PUB-190521-10
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 180: 396-402 (Journal)
- Registered Authors
- Yan, Bing
- Keywords
- Chicoric acid, Oxidative stress, Pb(2+) poisoning, Toxicity alleviation
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Caffeic Acids/pharmacology*
- Cell Line
- Cell Survival/drug effects
- Environmental Pollutants/toxicity*
- Heme Oxygenase-1/metabolism
- Larva/drug effects*
- Larva/metabolism
- Lead/toxicity*
- Mice
- Microglia/drug effects*
- Oxidative Stress/drug effects*
- Reactive Oxygen Species/metabolism
- Succinates/pharmacology*
- Toxicity Tests
- Zebrafish/metabolism*
- PubMed
- 31108416 Full text @ Ecotoxicol. Environ. Saf.
Citation
Mu, Y., Yu, J., Ji, W., Chen, L., Wang, X., Yan, B. (2019) Alleviation of Pb2+ pollution-induced oxidative stress and toxicity in microglial cells and zebrafish larvae by chicoric acid. Ecotoxicology and environmental safety. 180:396-402.
Abstract
Pb2+ pollution and poisoning are serious environmental and pharmacological concerns. The World Health Organization reported that Pb has resulted in 540,000 deaths in 2016 alone. Therefore, effective drugs or supplements that can alleviate or offset Pb2+-induced toxicity are badly needed. Through screening biocompatible natural compounds, we discovered that chicoric acid exhibited potent protective activities against Pb2+-induced toxicity both in BV-2 microglial cells and in zebrafish from the first days of development. Chicoric acid was able to reduce Pb2+-induced increases in levels of reactive oxygen species and tumor necrosis factor alpha, restoring the cell cycle in BV-2 cells. In the zebrafish model, chicoric acid significantly alleviated the Pb2+-induced serious mortality and malformation of zebrafish larvae in a concentration-dependent manner. These protective activities of chicoric acid were mainly from its alleviation of Pb2+-induced dysregulation of oxidative response pathways, including key genes such as Aox1, Gclm, Hmox1, Nqo1, Scd1, and Srxn1, as well as HO-1 protein. Since Pb2+ is difficult to be completely eliminated from the body and chelating agents may cause serious adverse effects, chicoric acid is likely a potential supplement therapy, in addition to current clinical practices.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping