PUBLICATION
Germline Structural Variations Are Preferential Sites of DNA Replication Timing Plasticity during Development
- Authors
- Hulke, M.L., Siefert, J.C., Sansam, C.L., Koren, A.
- ID
- ZDB-PUB-190512-6
- Date
- 2019
- Source
- Genome biology and evolution 11(6): 1663-1678 (Journal)
- Registered Authors
- Sansam, Chris, Siefert, Joseph
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Copy Number Variations
- DNA Replication*
- Embryo, Nonmammalian/metabolism
- Mutation Rate*
- Polymorphism, Single Nucleotide
- Time Factors
- Zebrafish/genetics*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- PubMed
- 31076752 Full text @ Genome Biol. Evol.
Citation
Hulke, M.L., Siefert, J.C., Sansam, C.L., Koren, A. (2019) Germline Structural Variations Are Preferential Sites of DNA Replication Timing Plasticity during Development. Genome biology and evolution. 11(6):1663-1678.
Abstract
The DNA replication timing program is modulated throughout development and is also one of the main factors influencing the distribution of mutation rates across the genome. However, the relationship between the mutagenic influence of replication timing and its developmental plasticity remains unexplored. Here, we studied the distribution of copy number variations (CNVs) and single nucleotide polymorphisms (SNPs) across the zebrafish genome in relation to changes in DNA replication timing during embryonic development in this model vertebrate species. We show that CNV sites exhibit strong replication timing plasticity during development, replicating significantly early during early development but significantly late during more advanced developmental stages. Reciprocally, genomic regions that changed their replication timing during development contained a higher proportion of CNVs than developmentally-constant regions. Developmentally-plastic CNV sites, in particular those that become delayed in their replication timing, were enriched for the clustered protocadherins, a set of genes important for neuronal development that have undergone extensive genetic and epigenetic diversification during zebrafish evolution. In contrast, SNP sites replicated consistently early throughout embryonic development, highlighting a unique aspect of the zebrafish genome. Our results uncover a hitherto unrecognized interface between development and evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping