PUBLICATION

Zebrafish as a Model to Study Cholestatic Liver Diseases

Authors
Pham, D.H., Yin, C.
ID
ZDB-PUB-190425-14
Date
2019
Source
Methods in molecular biology (Clifton, N.J.)   1981: 273-289 (Chapter)
Registered Authors
Yin, Chunyue
Keywords
BODIPY fluorescent fatty acid analog, Bile canaliculi, Bile duct, Bile salt transporters, Cholangiocyte, Confocal microscopy, Fluorescent bile acid derivative, Hepatocyte, Transmission electron microscopy
MeSH Terms
  • Animals
  • Bile Ducts/metabolism
  • Bile Ducts/pathology
  • Cholestasis/metabolism
  • Cholestasis/pathology*
  • Disease Models, Animal*
  • Liver/metabolism
  • Liver/pathology
  • Liver Diseases/metabolism
  • Liver Diseases/pathology*
  • Zebrafish*
PubMed
31016661 Full text @ Meth. Mol. Biol.
Abstract
Cholestasis is a condition that impairs bile flow, resulting in retention of bile fluid in the liver. It may cause significant morbidity and mortality due to pruritus, malnutrition, and complications from portal hypertension secondary to biliary cirrhosis. The zebrafish (Danio rerio) has emerged as a valuable model organism for studying cholestasis that complements with the in vitro systems and rodent models. Its main advantages include conserved mechanisms of liver development and bile formation, rapid external development, ease of monitoring hepatobiliary morphology and function in live larvae, and accessibility to genetic and chemical manipulations. In this chapter, we provide an overview of the existing zebrafish models of cholestatic liver diseases. We discuss the strengths and limitations of using zebrafish to study cholestasis. We also provide step-by-step descriptions of the methodologies for analyzing cholestatic phenotypes in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping