PUBLICATION
Exploring the Antiangiogenic Potential of Solomonamide A Bioactive Precursors: In Vitro and in Vivo Evidences of the Inhibitory Activity of Solo F-OH During Angiogenesis
- Authors
- Carrillo, P., Martínez-Poveda, B., Cheng-Sánchez, I., Guerra, J., Tobia, C., López-Romero, J.M., Sarabia, F., Medina, M.Á., Quesada, A.R.
- ID
- ZDB-PUB-190418-13
- Date
- 2019
- Source
- Marine drugs 17(4): (Journal)
- Registered Authors
- Tobia, Chiara
- Keywords
- Solomonamide A, angiogenesis, cancer, endothelial cells, marine sponge
- MeSH Terms
-
- Angiogenesis Inhibitors/chemistry
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Cell Culture Techniques
- Cell Line, Tumor
- Cell Movement/drug effects
- Cell Proliferation/drug effects
- Cell Survival/drug effects
- Chorioallantoic Membrane
- Endothelial Cells/drug effects
- Humans
- Inhibitory Concentration 50
- MAP Kinase Signaling System/drug effects
- Matrix Metalloproteinase 2/metabolism
- Matrix Metalloproteinase 9/metabolism
- Molecular Structure
- Oncogene Protein v-akt/metabolism
- Peptide Fragments/metabolism
- Peptides, Cyclic/chemistry
- Peptides, Cyclic/pharmacology*
- Signal Transduction/drug effects
- Zebrafish
- PubMed
- 30991727 Full text @ Mar. Drugs
Citation
Carrillo, P., Martínez-Poveda, B., Cheng-Sánchez, I., Guerra, J., Tobia, C., López-Romero, J.M., Sarabia, F., Medina, M.Á., Quesada, A.R. (2019) Exploring the Antiangiogenic Potential of Solomonamide A Bioactive Precursors: In Vitro and in Vivo Evidences of the Inhibitory Activity of Solo F-OH During Angiogenesis. Marine drugs. 17(4).
Abstract
Marine sponges are a prolific source of bioactive compounds. In this work, the putative antiangiogenic potential of a series of synthetic precursors of Solomonamide A, a cyclic peptide isolated from a marine sponge, was evaluated. By means of an in vitro screening, based on the inhibitory activity of endothelial tube formation, the compound Solo F-OH was selected for a deeper characterization of its antiangiogenic potential. Our results indicate that Solo F-OH is able to inhibit some key steps of the angiogenic process, including the proliferation, migration, and invasion of endothelial cells, as well as diminish their capability to degrade the extracellular matrix proteins. The antiangiogenic potential of Solo F-OH was confirmed by means of two different in vivo models: the chorioallantoic membrane (CAM) and the zebrafish yolk membrane (ZFYM) assays. The reduction in ERK1/2 and Akt phosphorylation in endothelial cells treated with Solo F-OH denotes that this compound could target the upstream components that are common to both pathways. Taken together, our results show a new and interesting biological activity of Solo F-OH as an inhibitor of the persistent and deregulated angiogenesis that characterizes cancer and other pathologies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping