PUBLICATION
The effects of Roundup® in embryo development and energy metabolism of the zebrafish (Danio rerio)
- Authors
- Panetto, O.S., Gomes, H.F., Fraga Gomes, D.S., Campos, E., Romeiro, N.C., Costa, E.P., do Carmo, P.R.L., Feitosa, N.M., Moraes, J.
- ID
- ZDB-PUB-190416-5
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 222: 74-81 (Journal)
- Registered Authors
- Feitosa, Natalia Martins
- Keywords
- Energy metabolism, Molecular docking, Roundup®, Toxicology, Zebrafish embryo
- MeSH Terms
-
- Animals
- Binding Sites
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Energy Metabolism/drug effects*
- Gene Expression Regulation, Enzymologic/drug effects
- Glucokinase/metabolism
- Glycine/administration & dosage
- Glycine/analogs & derivatives*
- Glycine/toxicity
- Hexokinase/metabolism
- Molecular Docking Simulation
- Molecular Structure
- Protein Conformation
- Zebrafish/embryology*
- PubMed
- 30981909 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Panetto, O.S., Gomes, H.F., Fraga Gomes, D.S., Campos, E., Romeiro, N.C., Costa, E.P., do Carmo, P.R.L., Feitosa, N.M., Moraes, J. (2019) The effects of Roundup® in embryo development and energy metabolism of the zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 222:74-81.
Abstract
Roundup® is currently the most widely used and sold agricultural pesticide in the world. The objective of this work was to investigate the effects of Roundup® on energy metabolism during zebrafish (Danio rerio) embryogenesis. The embryo toxicity test was performed for 96 h post-fertilisation and the sublethal concentration of Roundup® was defined as 58.3 mg/L, which resulted in failure to inflate the swim bladder. Biochemical assays were performed with viable embryos following glyphosate exposure, and no significant effects on protein, glucose, glycogen, triglyceride levels or the enzymatic activities of alanine aminotransferase and aspartate aminotransferase were observed. However, the activity of hexokinase was significantly altered following exposure to 11.7 mg/L Roundup®. Through molecular docking we have shown for the first time that the interactions of glucokinase and hexokinases 1 and 2 with glyphosate showed significant interactions in the active sites, corroborating the biochemical results of hexokinase activity in zebrafish exposed to the chemical. From the results of molecular docking interactions carried out on the Zfishglucok, ZfishHK1 and ZfishHK2 models with the glyphosate linker, it can be concluded that there are significant interactions between glyphosate and active sites of glucokinase and hexokinase 1 and 2 proteins. The present work suggests that Roundup® can induce problems in fish embryogenesis relating to the incapacity of swim bladder to inflate. This represents the first study demonstrating the interaction of glyphosate with hexokinase and its isoforms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping